Kimura S
Arch Dermatol Res (1975). 1977 Aug 22;259(2):161-8. doi: 10.1007/BF00557957.
In an attempt to elucidate the mechanism underlying the formation of cutaneous horns, 43 cases of cutaneous horns were examined histologically and histochemically. Three of 43 cases were also investigated by using direct immunofluorescence technique and electron microscopy. The structure identical to "Hornmark" was found in all of 43 cases of cutaneous horns except two which consisted only of horny masses. The substance found in the intercellular space of "Hornmark" was homogenous, eosinophilic and mostly diastase-resistant PAS positive, and showed strongly positive immunofluorescence for immunoglobulins, complement, and fibrinogen. Electron microscopically, it was moderately electron dense, fine granular and in part fine fibrillar, and was similar to the contents of capillary lumen. From these findings, it was suggested that the substance found in "Hornmark" consisted mainly of the components of plasma protein, and that coagulated plasma protein might play an important role on the increased cohesiveness of the horny cells, i.d. on the formation of cutaneous horns.
为了阐明皮肤角形成的潜在机制，对43例皮肤角进行了组织学和组织化学检查。43例中的3例还采用直接免疫荧光技术和电子显微镜进行了研究。除2例仅由角质块组成的皮肤角外，在其余43例皮肤角中均发现了与“角标记”相同的结构。在“角标记”的细胞间隙中发现的物质均匀、嗜酸性，大多对淀粉酶抵抗的PAS阳性，并且对免疫球蛋白、补体和纤维蛋白原显示强阳性免疫荧光。在电子显微镜下，它具有中等电子密度，呈细颗粒状，部分呈细纤维状，与毛细血管腔内容物相似。从这些发现推测，在“角标记”中发现的物质主要由血浆蛋白成分组成，并且凝固的血浆蛋白可能对角质形成细胞凝聚力的增加起重要作用，即对皮肤角的形成起重要作用。
