Cardenas D D, Stolov W C, Hardy R
Arch Phys Med Rehabil. 1977 Oct;58(10):423-6.
Eight Sprague-Dawley rats 10 to 12 weeks of age had one limb immobilized by plaster in knee flexion and ankle plantar flexion for four weeks. The soleus muscles from the control and immobilized limbs were excised and frozen. The technique used insured that cross sections subsequently cut and stained would contain all the fibers extending from the origin to the insertion aponeuroses. The total number of muscle fibers per muscle was counted from 25 to 50 photomicrographs made for each muscle cross section. Paired comparisons of total muscle fibers in control and immobilized muscles revealed no significant differences. The total number of fibers in control and immobilized soleus muscles was 2812 +/- 521 and 2930 +/- 403, respectively. Therefore, muscle fiber loss does not occur in disuse atrophy produced by external immobilization as has been reported.
8只10至12周龄的斯普拉格-道利大鼠，将其一肢用石膏固定于膝关节屈曲和踝关节跖屈位4周。切除对照肢体和固定肢体的比目鱼肌并冷冻。所采用的技术确保随后切割和染色的横截面将包含从起点延伸至止点腱膜的所有纤维。从每个肌肉横截面制作的25至50张显微照片中计算每块肌肉的肌纤维总数。对照肌肉和固定肌肉中总肌纤维的配对比较显示无显著差异。对照比目鱼肌和固定比目鱼肌中的纤维总数分别为2812±521和2930±403。因此，如所报道的那样，在外部固定导致的废用性萎缩中不会发生肌纤维丢失。
