Belkhiri A, Intengan H, Klassen G R
Department of Microbiology, University of Manitoba, Winnipeg, Canada.
Gene. 1997 Feb 28;186(2):155-9. doi: 10.1016/s0378-1119(96)00662-2.
The 5S ribosomal RNA genes of the oomycete Pythium irregulare exist in tandem arrays unlinked to the rDNA repeat unit. A clone with a 9.2-kb insert containing an array of 5S genes was identified in a lambda genomic library and was characterized by restriction mapping and partial sequencing. The array consisted of 9 apparently identical 5S genes and their spacers in tandem, followed by a diverged 5S-like sequence that is likely to be a pseudogene. This gene arrangement, although almost universal in plants and animals, is rare in fungi and protists.
低等卵菌不规则腐霉的5S核糖体RNA基因以串联阵列形式存在，与核糖体DNA重复单元不相连。在一个λ基因组文库中鉴定出一个含有9.2 kb插入片段且包含5S基因阵列的克隆，并通过限制性酶切图谱分析和部分测序对其进行了表征。该阵列由9个明显相同的5S基因及其间隔区串联组成，后面跟着一个可能是假基因的差异5S样序列。这种基因排列方式虽然在植物和动物中几乎普遍存在，但在真菌和原生生物中却很少见。
