大鼠5S rRNA真实基因重复序列定位于19号染色体的19q12至qter区域，假基因重复序列定位于12号染色体的12q12区域。

The rat 5S rRNA bona fide gene repeat maps to chromosome 19q12-->qter and the pseudogene repeat maps to 12q12.

作者信息

Frederiksen S, Cao H, Lomholt B, Levan G, Hallenberg C

机构信息

Biochemistry Laboratory B, Panum Institute, University of Copenhagen, Denmark. sune biobase.dk

出版信息

Cytogenet Cell Genet. 1997;76(1-2):101-6. doi: 10.1159/000134523.

DOI:10.1159/000134523

PMID:9154136

Abstract

The bona fide 5S rRNA genes in the rat are found in a 1.8-kb tandem repeat and the pseudogenes occur in a 2.5-kb tandem repeat. Three bona fide 5S rRNA genes and one gene variant with one base substitution in the coding region were isolated from the 1.8-kb repeat. Six pseudogenes were isolated from the 2.5-kb repeat. The total number of genes/gene variants/pseudogenes is 700-1200 copies per haploid genome, and the pseudogene repeat contains about 50% more 5S rDNA related sequences compared with the bona fide gene repeat. Various well-defined 5' - and 3'-flanking sequences of the bona fide gene and of the pseudogene were used for in situ hybridization to metaphase chromosomes. The results showed that the bona fide 5S rRNA gene repeat Rn5s maps to chromosome 19q12 and the pseudogene repeat Rn5sp maps to 12q12.

摘要

大鼠中真正的5S rRNA基因存在于一个1.8kb的串联重复序列中，而假基因则存在于一个2.5kb的串联重复序列中。从1.8kb的重复序列中分离出了三个真正的5S rRNA基因和一个编码区有一个碱基替换的基因变体。从2.5kb的重复序列中分离出了六个假基因。每个单倍体基因组中基因/基因变体/假基因的总数为700 - 1200个拷贝，与真正的基因重复序列相比，假基因重复序列包含的5S rDNA相关序列多约50%。真正基因和假基因的各种明确的5' - 和3' - 侧翼序列被用于中期染色体的原位杂交。结果表明，真正的5S rRNA基因重复序列Rn5s定位于19号染色体q12，假基因重复序列Rn5sp定位于12号染色体q12。