Satoh A K, Tokunaga F, Ozaki K
Department of Biology, Graduate School of Science, Osaka University, Japan.
FEBS Lett. 1997 Mar 3;404(1):65-9. doi: 10.1016/s0014-5793(97)00094-x.
From a Drosophila head cDNA library, we isolated 9 cDNA clones, each of which encodes a different member of Rab-protein family. Seven of them (DRabs) have more than 80% amino acid identity to the corresponding members of mammalian Rab proteins. The other two proteins (DRabRP3 and 4) show low sequence similarity to any of the known Rab proteins. However, both contain all amino acids conserved in known Rabs. In addition, DRabRP4 has strong GTP-binding activity, when synthesized in E. coli cells. These results indicate that DRabRPs are novel members of the Rab-protein family. Molecular phylogenetic analysis also supported this conclusion.
我们从果蝇头部cDNA文库中分离出9个cDNA克隆，每个克隆编码Rab蛋白家族的一个不同成员。其中7个（DRabs）与哺乳动物Rab蛋白的相应成员具有80%以上的氨基酸同一性。另外两个蛋白（DRabRP3和4）与任何已知的Rab蛋白的序列相似性较低。然而，两者都含有已知Rab蛋白中保守的所有氨基酸。此外，当在大肠杆菌细胞中合成时，DRabRP4具有很强的GTP结合活性。这些结果表明DRabRPs是Rab蛋白家族的新成员。分子系统发育分析也支持这一结论。
