[Method of stereometric study of the intramural arterial bed of the walls of the human heart ventricles].

Principles and technique for selecting material from the human heart ventricular walls to study stereometrically their intramural arterial bed are described. The left ventricular wall was investigated as an example. Stereometric investigation of the intramural arterial bed of the myocardium is carried out under an ocular grid by the method of dots counting on histological sections, obtained from pieces of three-plane orientation (horizontal, vertical and frontal). To obtain the most informative data on average volume density of the intramural arterial bed along the whole left ventricular wall, it is enough to confine our examination to its six areas (superior, medial, inferior thirds of the anteior and posterior-lateral parts of the left ventricular wall). From each area, at least, one piece with horizontal and vertical and two pieces with frontal orientation should be taken. In order to obtain reliable data on volume density of the intramural bed in a certain myocardial part (a zone of infarction, cardiosclerosis, etc.), it is necessary to take more pieces excised, no less than two both with horizontal and vertical orientation and four with frontal orientation.