McKeon T A, Goodrich-Tanrikulu M, Lin J T, Stafford A
USDA, ARS, Western Regional Research Center, Albany, California 94710, USA.
Lipids. 1997 Jan;32(1):1-5. doi: 10.1007/s11745-997-0001-8.
Neurospora crassa incorporated exogenous deuterated palmitate (16:0) and 14C-labeled oleate (18:1 delta 9) into cell lipids. Of the exogenous 18:1 delta 9 incorporated, 59% was desaturated to 18:2 delta 9,12 and 18:3 delta 9,12,15. Of the exogenous 16:0 incorporated, 20% was elongated to 18:0, while 37% was elongated and desaturated into 18:1 delta 9, 18:2 delta 9,12, and 18:3 delta 9,12,15. The mass of unsaturated fatty acids in phospholipid and triacylglycerol is 12 times greater than the mass of 18:0. Deuterium label incorporation in unsaturated fatty acids is only twofold greater than in 18:0, indicating a sixfold preferential use of 16:0 for saturated fatty acid synthesis. These results indicate that the release of 16:0 from fatty acid synthase is a key control point that influences fatty acid composition in Neurospora.
粗糙脉孢菌将外源性氘代棕榈酸（16:0）和14C标记的油酸（18:1 Δ9）掺入细胞脂质中。在外源性掺入的18:1 Δ9中，59%被去饱和为18:2 Δ9,12和18:3 Δ9,12,15。在外源性掺入的16:0中，20%被延长为18:0，而37%被延长并去饱和为18:1 Δ9、18:2 Δ9,12和18:3 Δ9,12,15。磷脂和三酰甘油中不饱和脂肪酸的质量比18:0的质量大12倍。不饱和脂肪酸中氘标记的掺入仅比18:0中的掺入大两倍，表明在饱和脂肪酸合成中对16:0的优先使用是六倍。这些结果表明，脂肪酸合酶释放16:0是影响粗糙脉孢菌脂肪酸组成的关键控制点。
