Measurement of extravascular lung water in dogs using the thermal-green dye indicator dilution method.

The measurement of extravascular lung water by a double-indicator dilution technique using cold indocyanine green dye was evaluated in dogs. Pulmonary edema was induced in 11 animals by volume overload; 12 animals served as controls. For each measurement, the two indicators (cold dye) were injected into the superior vena cava and detected in the femoral artery. The extravascular thermal volume was calculated using the mean transit times of the two indicator curves. Pretermination measurements of extravascular thermal volume correlated closely with standard gravimetric analysis of pulmonary extravascular tissue weight (EVTV - 1.15 PEW + 2.1 ml/kg, n = 21, r = 0.97, P less than 0.001; where EVTV = extravascular thermal volume and PEW = pulmonary extravascular tissue weight). Throughout the experiment, the arterial oxygen tension and alveolar-arterial oxygen tension gradient, correlated poorly with EVTV (linear correlation: r = 0.47, 0.45, respectively). The intrapulmonary shunt correlated better with EVTV (r = 0.72). Nonlinear correlation of EVTV with intravascular pressures (left ventricular filling pressures, colloid oncotic pressures, and the pulmonary artery occlusion pressure-colloid oncotic pressure gradient) were more significant than linear relationships. The critical pressures at which lung water rapidly increased in this model occurred at left ventricular filling pressures of 22-27 mmHg and at pulmonary artery occlusion pressure-colloid oncotic pressure gradients of 25-30 mmHg. The thermal dye technique appears to provide an accurate measurement of lung water changes in this pressure edema model.