Olins A L, Olins D E, Bazett-Jones D P
University of Tennessee-Oak Ridge Graduate School of Biomedical Sciences, Oak Ridge National Laboratory 37831-8080, USA.
Biol Cell. 1996;87(3):143-7.
Ultra-thin sections of Chironomus salivary glands were stained in a non-Feulgen procedure with osmium ammine-B and imaged at several electron energy-loss windows. For two types of RNP-containing structures (ie Balbiani ring granules and endoplasmic reticulum), a significant spatial correlation was observed between stain distribution and net phosphorus distribution. Non-Feulgen osmium ammine-B staining does not require the use of ultra-thin sections and can approximate the distribution of nucleic acid phosphorus.
摇蚊唾液腺的超薄切片采用锇氨-B非福尔根染色法进行染色，并在多个电子能量损失窗口成像。对于两种含核糖核蛋白的结构（即巴尔比亚尼环颗粒和内质网），在染色剂分布和净磷分布之间观察到显著的空间相关性。非福尔根锇氨-B染色不需要使用超薄切片，并且可以近似核酸磷的分布。
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