• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利:CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法,用中文像聊天一样搜索,搜遍4000万医学文献。AI智能推荐,让科研检索更轻松。

立即免费搜索

文件翻译

保留排版,准确专业,支持PDF/Word/PPT等文件格式,支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述,25分钟生成高质量综述,智能提取关键信息,辅助科研写作。

立即免费体验

Synthesis of a more stable osmium ammine electron-dense DNA stain.

作者信息

Olins A L, Moyer B A, Kim S H, Allison D P

机构信息

University of Tennessee-Oak Ridge, Graduate School of Biomedical Sciences.

出版信息

J Histochem Cytochem. 1989 Mar;37(3):395-8. doi: 10.1177/37.3.2465337.

DOI:10.1177/37.3.2465337
PMID:2465337
Abstract

Specific DNA staining for electron microscopic observation is simplified by a shorter synthesis of the staining reagent. The new, more reliable reagent, osmium ammine-B, is stable for more than a year, dissolves completely in water, and does not require reoptimization of staining conditions for every batch, yet reproducibly gives strong contrast to DNA-containing structures.

摘要

相似文献

1
Synthesis of a more stable osmium ammine electron-dense DNA stain.
J Histochem Cytochem. 1989 Mar;37(3):395-8. doi: 10.1177/37.3.2465337.
2
Osmium Ammine for Staining DNA in Electron Microscopy.用于电子显微镜中DNA染色的锇氨络合物
Methods Mol Biol. 2017;1560:261-267. doi: 10.1007/978-1-4939-6788-9_19.
3
Activation of osmium ammine by SO2-generating chemicals for EM Feulgen-type staining of DNA.通过产生二氧化硫的化学物质激活锇胺用于DNA的电子显微镜福尔根氏染色。
Eur J Histochem. 1995;39(2):101-6.
4
Osmium ammine-B and electron spectroscopic imaging of ribonucleoproteins: correlation of stain and phosphorus.锇氨络合物B与核糖核蛋白的电子光谱成像:染色与磷的相关性
Biol Cell. 1996;87(3):143-7.
5
An alternative method for preparation of Schiff-like reagent from osmium-ammine complex for selective staining of DNA on thin Lowicryl sections.一种由锇-氨络合物制备席夫样试剂的替代方法,用于在超薄Lowicryl切片上对DNA进行选择性染色。
J Histochem Cytochem. 1994 Dec;42(12):1643-9. doi: 10.1177/42.12.7983365.
6
Distribution of DNA, nuclear micro-heterogeneities and compaction of the chromatin in rabbit epididymal spermatozoa. Ultrastructural evaluation of the Feulgen-like technique using osmium ammine.兔附睾精子中DNA的分布、核微异质性及染色质的压缩。使用锇胺对类福尔根技术进行超微结构评估。
Reprod Nutr Dev. 1994;34(3):261-72. doi: 10.1051/rnd:19940308.
7
Osmium ammine: review of current applications to visualize DNA in electron microscopy.锇氨配合物:电子显微镜下可视化DNA的当前应用综述。
Biol Cell. 1996;87(3):121-32.
8
DNA changes involved in the formation of metaphase chromosomes, as observed in mouse duodenal crypt cells stained by osmium-ammine. II. Tracing nascent DNA by bromodeoxyuridine into structures arising during the S phase.在经锇氨染色的小鼠十二指肠隐窝细胞中观察到的中期染色体形成过程中涉及的DNA变化。II. 用溴脱氧尿苷追踪新生DNA进入S期形成的结构。
Anat Rec. 1995 Aug;242(4):449-61. doi: 10.1002/ar.1092420403.
9
Selective staining of nucleic acids by osmium-ammine complex in thin sections from lowicryl-embedded samples.
J Histochem Cytochem. 1990 Oct;38(10):1495-501. doi: 10.1177/38.10.2205645.
10
Visualization of DNA within mitochondria by osmium-ammine staining of mouse duodenal crypt cells.通过对小鼠十二指肠隐窝细胞进行锇胺染色观察线粒体中的DNA。
J Cell Sci. 1992 Apr;101 ( Pt 4):785-93. doi: 10.1242/jcs.101.4.785.

引用本文的文献

1
ChromEMT: visualizing and reconstructing chromatin ultrastructure and 3D organization in situ.染色体电子显微镜断层成像技术:原位可视化和重建染色质超微结构及三维组织
Nat Protoc. 2025 Apr;20(4):934-966. doi: 10.1038/s41596-024-01071-2. Epub 2024 Nov 29.
2
A brief history of the Feulgen reaction.费林反应简史。
Histochem Cell Biol. 2024 Jul;162(1-2):3-12. doi: 10.1007/s00418-024-02279-9. Epub 2024 Apr 12.
3
The Feulgen reaction at the electron microscopy level.电子显微镜水平的福尔根反应。
Eur J Histochem. 2024 Feb 23;68(1):3989. doi: 10.4081/ejh.2024.3989.
4
Dinuclear nitrido-bridged osmium complexes inhibit the mitochondrial calcium uniporter and protect cortical neurons against lethal oxygen-glucose deprivation.双核氮桥联锇配合物抑制线粒体钙单向转运体并保护皮层神经元免受致死性氧糖剥夺损伤。
RSC Chem Biol. 2022 Nov 14;4(1):84-93. doi: 10.1039/d2cb00189f. eCollection 2023 Jan 4.
5
Histochemistry for nucleic acid research: 60 years in the European Journal of Histochemistry.核酸研究的组织化学:《欧洲组织化学杂志》60 年。
Eur J Histochem. 2022 Apr 20;66(2):3409. doi: 10.4081/ejh.2022.3409.
6
Nuclear Compartments: An Incomplete Primer to Nuclear Compartments, Bodies, and Genome Organization Relative to Nuclear Architecture.核区:核区、核体以及基因组组织相对于核架构的不完全入门指南。
Cold Spring Harb Perspect Biol. 2022 Jul 1;14(7):a041268. doi: 10.1101/cshperspect.a041268.
7
Correlative Light and Electron Microscopy (CLEM) Analysis of Nuclear Reorganization Induced by Clustered DNA Damage Upon Charged Particle Irradiation.带电粒子辐照诱导的 DNA 簇损伤导致的核重排的相关光镜和电子显微镜分析。
Int J Mol Sci. 2020 Mar 11;21(6):1911. doi: 10.3390/ijms21061911.
8
Epichromatin and chromomeres: a 'fuzzy' perspective.常染色质和异染色质:一种“模糊”的观点。
Open Biol. 2018 Jun;8(6). doi: 10.1098/rsob.180058.
9
ChromEMT: Visualizing 3D chromatin structure and compaction in interphase and mitotic cells.染色体电子显微镜断层扫描技术(ChromEMT):可视化间期和有丝分裂细胞中的三维染色质结构与压缩状态
Science. 2017 Jul 28;357(6349). doi: 10.1126/science.aag0025.
10
Chromatin structure in situ: the contribution of DNA ultrastructural cytochemistry.原位染色质结构:DNA超微结构细胞化学的贡献。
Eur J Histochem. 2014 Jan 24;58(1):2307. doi: 10.4081/ejh.2014.2307.