Matsukawa Y, Nishino H, Okuyama Y, Matsui T, Matsumoto T, Matsumura S, Shimizu Y, Sowa Y, Sakai T
Department of Preventive Medicine, Kyoto Prefectural University of Medicine, Kamikyo-ku, Japan.
Oncology. 1997 Mar-Apr;54(2):118-21. doi: 10.1159/000227674.
The present study examines the effect of dietary quercetin and/or restraint stress on the formation of aberrant crypt foci (ACF) induced by azoxymethane (AOM) in the colon. Female F344 rats were divided into four groups. All animals were given intraperitoneal injections of AOM. The animals were fed a basal diet (group A, C), or a 2% quercetin-supplemented diet (group B, D). The animals were put individually to narrow wire cages for 1 h every day throughout the study to expose them to mild restraint stress (group C, D). At week 5, all the rats were killed and analyzed for ACF in the colon. The number of ACF increased significantly in the animals subjected to stress (p < 0.05). On the other hand, dietary quercetin significantly reduced the number of ACF in both the nonstress (p < 0.001) and stress groups (p < 0.05). These findings suggest that quercetin might have a potential as a chemopreventive drug for colon cancer despite stress.
本研究考察了膳食槲皮素和/或束缚应激对结肠中由氧化偶氮甲烷（AOM）诱导产生的异常隐窝灶（ACF）形成的影响。将雌性F344大鼠分为四组。所有动物均腹腔注射AOM。给动物喂食基础饲料（A组、C组），或添加2%槲皮素的饲料（B组、D组）。在整个研究过程中，每天将动物单独置于狭窄的铁丝笼中1小时，使其受到轻度束缚应激（C组、D组）。在第5周时，处死所有大鼠并分析结肠中的ACF。受到应激的动物中ACF数量显著增加（p<0.05）。另一方面，膳食槲皮素在无应激组（p<0.001）和应激组（p<0.05）中均显著减少了ACF的数量。这些发现表明，尽管存在应激，槲皮素可能具有作为结肠癌化学预防药物的潜力。
