Onogi N, Okuno M, Komaki C, Moriwaki H, Kawamori T, Tanaka T, Mori H, Muto Y
First Department of Internal Medicine, Gifu University School of Medicine, Japan.
Carcinogenesis. 1996 Jun;17(6):1291-6. doi: 10.1093/carcin/17.6.1291.
We have investigated the modulatory effect of dietary perilla oil which is rich in the n-3 polyunsaturated fatty acid, alpha-linolenic acid, on the development of azoxymethane (AOM)-induced colonic aberrant crypt foci (ACF) in male F344 rats. Animals were given three weekly subcutaneous injections of AOM (15 mg/kg body weight) to induce ACE. The rats were fed a basal diet containing either 12% olive oil, 12% safflower oil, 12% perilla oil, 6% perilla oil plus 6% olive oil, or 3% perilla oil plus 9% olive oil for 5 weeks, starting 1 week before the first dosing of AOM. All rats were sacrificed 2 weeks after the last AOM injection. The amount of food consumed and body weight gain were identical among every dietary group. The frequency of ACF was significantly lower in the rats fed 12% perilla oil than in those fed 12% olive oil or 12% safflower oil (P < 0.01 and P < 0.05, respectively). The suppressive effect of perilla oil was dose-dependent, as the number of ACF was 20.7, 40.7 and 47.4% of those of the 12% olive oil-fed controls in rats fed 12% perilla oil, 6% perilla oil plus 6% olive oil and 3% perilla oil plus 9% olive oil, respectively. Perilla oil significantly reduced ras expression as well as the AgNORs count (cell proliferation biomarkers) in the colonic mucosa, as compared with olive oil or safflower oil (P < 0.01, respectively). Marked increases in n-3 polyunsaturated fatty acids in membrane phospholipid fractions and decreased PGE2 levels were observed in colonic mucosa of perilla oil-fed rats. These results suggest that perilla oil, even in small amounts, suppresses the development of aberrant crypt foci, and is therefore a possible preventive agent in the early stage of colon carcinogenesis.
我们研究了富含n-3多不饱和脂肪酸α-亚麻酸的紫苏油对雄性F344大鼠中由氧化偶氮甲烷（AOM）诱导的结肠异常隐窝灶（ACF）形成的调节作用。给动物每周皮下注射三次AOM（15毫克/千克体重）以诱导ACF。从首次给予AOM前1周开始，大鼠被喂食含12%橄榄油、12%红花油、12%紫苏油、6%紫苏油加6%橄榄油或3%紫苏油加9%橄榄油的基础饮食5周。在最后一次注射AOM后2周处死所有大鼠。每个饮食组的食物消耗量和体重增加量相同。喂食12%紫苏油的大鼠中ACF的频率显著低于喂食12%橄榄油或12%红花油的大鼠（分别为P < 0.01和P < 0.05）。紫苏油的抑制作用呈剂量依赖性，因为在喂食12%紫苏油、6%紫苏油加6%橄榄油和3%紫苏油加9%橄榄油的大鼠中，ACF的数量分别是喂食12%橄榄油对照组的20.7%、40.7%和47.4%。与橄榄油或红花油相比，紫苏油显著降低了结肠黏膜中ras表达以及嗜银核仁组织区计数（细胞增殖生物标志物）（分别为P < 0.01）。在喂食紫苏油的大鼠结肠黏膜中观察到膜磷脂组分中n-3多不饱和脂肪酸显著增加，前列腺素E2水平降低。这些结果表明，即使少量的紫苏油也能抑制异常隐窝灶的形成，因此可能是结肠癌发生早期的一种预防剂。
