Sarciron E M, Bresson-Hadni S, Mercier M, Lawton P, Duranton C, Lenys D, Petavy A F, Vuitton D A
Laboratory of Parasitology and Medical Mycology, Claude-Bernard University, Faculty of Pharmacy, Lyon, France.
Parasite Immunol. 1997 Feb;19(2):61-8. doi: 10.1046/j.1365-3024.1997.d01-183.x.
The immunological properties of the purified alkaline phosphatase (pAP) of Echinococcus multilocularis metacestodes have been investigated using alveolar echinococcosis (AE) patient sera in ELISA tests. A comparative study was done with EmC-Ag (crude antigen) and pAP-Ag (purified antigen). When the parasite purified enzyme pAP was used as antigen, the specificity of the ELISA was markedly increased since it reached 100% without any decrease of its sensitivity (100%). The serologic follow-up of AE patients was conducted during several months with these two antigens in three categories of patients: cured, stabilized and aggravated. There was a good correlation between clinical and serologic data when the pAP was used as antigen in ELISA tests. The anti-pAP antibodies titres did change more rapidly than anti-EmC antibodies titres when a recurrence occurred. Modifications of the anti-pAP antibodies levels were also observed during the patient's therapy: mebendazole, albendazole and Isoprinosine. These results suggest that pAP-Ag should be used for the diagnosis and the follow-up of AE patients.
利用泡型包虫病（AE）患者血清，通过酶联免疫吸附测定（ELISA）试验研究了多房棘球绦虫幼虫纯化碱性磷酸酶（pAP）的免疫特性。同时以EmC-Ag（粗抗原）和pAP-Ag（纯化抗原）进行了对比研究。当将寄生虫纯化酶pAP用作抗原时，ELISA的特异性显著提高，因为其特异性达到100%，且敏感性未降低（100%）。使用这两种抗原，对三类患者（治愈、病情稳定和病情加重）的AE患者进行了数月的血清学随访。在ELISA试验中，当使用pAP作为抗原时，临床和血清学数据之间存在良好的相关性。复发时，抗pAP抗体滴度的变化比抗EmC抗体滴度更快。在患者接受甲苯达唑、阿苯达唑和异丙肌苷治疗期间，也观察到了抗pAP抗体水平的变化。这些结果表明，pAP-Ag应用于AE患者的诊断和随访。
