尿嘧啶及取代尿嘧啶环氮质子的核磁共振光谱；与转运核糖核酸溶液结构中Aψ碱基配对的相关性
NMR spectroscopy of the ring nitrogen protons of uracil and substituted uracils; relevance to A psi base pairing in the solution structure of transfer RNA.
作者信息
Hurd R E, Reid B R
出版信息
Nucleic Acids Res. 1977 Aug;4(8):2747-55. doi: 10.1093/nar/4.8.2747.
Abstract
The chemical shifts of the well-resolved ring nitrogen protons of uracil and a series of substituted uracils, including pseudouridine (5-ribosyl-uracil) and uridine (1-ribosyl-uracil) were determined using nuclear magnetic resonance (NMR). The observed chemical shifts suggest the existence of an atypical syn conformation for pseudouridine in the Apsi base pair in regulatory tRNAs in solution.
摘要
利用核磁共振（NMR）测定了尿嘧啶以及一系列取代尿嘧啶（包括假尿苷（5-核糖基-尿嘧啶）和尿苷（1-核糖基-尿嘧啶））中分辨率良好的环氮质子的化学位移。观察到的化学位移表明，在溶液中调控性tRNA的Aψi碱基对中，假尿苷存在一种非典型的顺式构象。
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