Schejter E, Roy S, Sánchez V, Redfield A G
Nucleic Acids Res. 1982 Dec 20;10(24):8297-305. doi: 10.1093/nar/10.24.8297.
The proton NMR spectrum of yeast tRNAVal 1 has been studied using nuclear Overhauser effect (NOE), including comparison of NOE patterns between purine C8 deuterated and nondeuterated samples. Studies of the downfield region enable us to reliably assign many resonances in the acceptor and D stems. Prominent among these reliable assignments is that of the unusual base pair U psi, which is made here for the first time. Other identifications include GU2, U8-A14, the three AU base pairs of the acceptor stem, and N1 and N3 protons of psi 55.
已使用核Overhauser效应(NOE)研究了酵母tRNAVal 1的质子核磁共振谱,包括嘌呤C8氘代和非氘代样品之间NOE模式的比较。对低场区域的研究使我们能够可靠地归属受体茎和D茎中的许多共振峰。这些可靠归属中突出的是首次在此确定的异常碱基对Uψ。其他确定的归属包括GU2、U8-A14、受体茎的三个AU碱基对以及ψ55的N1和N3质子。