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1
Procedure for C2 deuteration of nucleic acids and determination of A psi 31 pseudouridine conformation by nuclear Overhauser effect in yeast tRNAPhe.酵母苯丙氨酸转运核糖核酸中核酸C2位氘代及通过核Overhauser效应测定ψ31假尿苷构象的方法
Nucleic Acids Res. 1982 Dec 20;10(24):8341-9. doi: 10.1093/nar/10.24.8341.
2
Nuclear Overhauser effect study of yeast tRNAVal 1: evidence for uridine-pseudouridine base pairing.酵母tRNAVal 1的核Overhauser效应研究：尿苷-假尿苷碱基配对的证据
Nucleic Acids Res. 1982 Dec 20;10(24):8297-305. doi: 10.1093/nar/10.24.8297.
3
Nuclear overhauser effect study of yeast aspartate transfer ribonucleic acid.
Biochemistry. 1982 Nov 23;21(24):6081-8. doi: 10.1021/bi00267a009.
4
Imino-proton resonances of yeast tRNAPhe studied by two-dimensional nuclear Overhauser enhancement spectroscopy.通过二维核Overhauser增强光谱法研究酵母苯丙氨酸转运核糖核酸的亚氨基质子共振。
Eur J Biochem. 1985 Jun 18;149(3):649-55. doi: 10.1111/j.1432-1033.1985.tb08973.x.
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Nuclear Overhauser effect study and assignment of D stem and reverse-Hoogsteen base pair proton resonances in yeast tRNAAsp.酵母天冬氨酸转运核糖核酸中D茎和反向-霍格施泰因碱基对质子共振的核Overhauser效应研究与归属
Nucleic Acids Res. 1981 Dec 21;9(24):7073-83. doi: 10.1093/nar/9.24.7073.
6
Nuclear Overhauser effect in specifically deuterated macromolecules: NMR assay for unusual base pairing in transfer RNA.特定氘代大分子中的核Overhauser效应：用于转运RNA中异常碱基配对的核磁共振分析
Proc Natl Acad Sci U S A. 1980 Oct;77(10):5659-62. doi: 10.1073/pnas.77.10.5659.
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15N-labeled Escherichia coli tRNAfMet, tRNAGlu, tRNATyr, and tRNAPhe. Double resonance and two-dimensional NMR of N1-labeled pseudouridine.15N标记的大肠杆菌甲硫氨酸起始tRNA、谷氨酸tRNA、酪氨酸tRNA和苯丙氨酸tRNA。N1标记假尿苷的双共振和二维核磁共振。
J Biol Chem. 1985 Aug 15;260(17):9734-41.
8
Major identity determinants for enzymatic formation of ribothymidine and pseudouridine in the T psi-loop of yeast tRNAs.酵母tRNA的Tψ环中核糖胸苷和假尿苷酶促形成的主要身份决定因素。
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9
Comparative conformations of uridine and pseudouridine and their derivatives.尿苷和假尿苷及其衍生物的比较构象
Eur J Biochem. 1980 Jul;108(2):457-63. doi: 10.1111/j.1432-1033.1980.tb04742.x.
10
Nuclear magnetic resonance and nuclear Overhauser effect study of yeast phenylalanine transfer ribonucleic acid imino protons.酵母苯丙氨酸转移核糖核酸亚氨基质子的核磁共振与核Overhauser效应研究
Biochemistry. 1981 Mar 3;20(5):1147-56. doi: 10.1021/bi00508a016.

引用本文的文献

1
Nuclear magnetic resonance studies on yeast tRNAPhe. III. Assignments of the iminoproton resonances of the tertiary structure by means of nuclear Overhauser effect experiments at 500 MHz.酵母苯丙氨酸转运核糖核酸的核磁共振研究。III. 通过500兆赫的核Overhauser效应实验对三级结构亚氨基质子共振进行归属
Nucleic Acids Res. 1983 Jul 11;11(13):4501-20. doi: 10.1093/nar/11.13.4501.
2
Nuclear magnetic resonance studies on yeast tRNAPhe. II. Assignment of the iminoproton resonances of the anticodon and T stem by means of nuclear Overhauser effect experiments at 500 MHz.酵母苯丙氨酸转运核糖核酸的核磁共振研究。II. 通过500兆赫的核Overhauser效应实验对反密码子和T茎的亚氨基质子共振进行归属。
Nucleic Acids Res. 1983 Jul 11;11(13):4483-99. doi: 10.1093/nar/11.13.4483.

本文引用的文献

1
Study of transfer ribonucleic acid unfolding by dynamic nuclear magnetic resonance.通过动态核磁共振研究转移核糖核酸的解折叠
Biochemistry. 1981 Jul 7;20(14):3996-4006. doi: 10.1021/bi00517a008.
2
Environment of ribothymidine in transfer ribonucleic acid studied by means of nuclear Overhauser effect.通过核Overhauser效应研究转移核糖核酸中核糖胸腺嘧啶的环境。
Biochemistry. 1981 Apr 14;20(8):2133-40. doi: 10.1021/bi00511a010.
3
Nuclear magnetic resonance and nuclear Overhauser effect study of yeast phenylalanine transfer ribonucleic acid imino protons.酵母苯丙氨酸转移核糖核酸亚氨基质子的核磁共振与核Overhauser效应研究
Biochemistry. 1981 Mar 3;20(5):1147-56. doi: 10.1021/bi00508a016.
4
Nuclear Overhauser effect in specifically deuterated macromolecules: NMR assay for unusual base pairing in transfer RNA.特定氘代大分子中的核Overhauser效应：用于转运RNA中异常碱基配对的核磁共振分析
Proc Natl Acad Sci U S A. 1980 Oct;77(10):5659-62. doi: 10.1073/pnas.77.10.5659.
5
Direct assignment of the dihydrouridine-helix imino proton resonances in transfer ribonucleic acid nuclear magnetic resonance spectra by means of the nuclear Overhauser effect.借助核Overhauser效应直接确定转移核糖核酸核磁共振谱中二氢尿嘧啶-螺旋亚氨基质子的共振峰
Biochemistry. 1982 Apr 13;21(8):1835-42. doi: 10.1021/bi00537a020.
6
DNA conformation, dynamics, and interactions in solution.溶液中的DNA构象、动力学及相互作用
Science. 1982 May 7;216(4546):581-90. doi: 10.1126/science.6280281.
7
Nuclear Overhauser effect study and assignment of D stem and reverse-Hoogsteen base pair proton resonances in yeast tRNAAsp.酵母天冬氨酸转运核糖核酸中D茎和反向-霍格施泰因碱基对质子共振的核Overhauser效应研究与归属
Nucleic Acids Res. 1981 Dec 21;9(24):7073-83. doi: 10.1093/nar/9.24.7073.
8
Mutant tRNA His ineffective in repression and lacking two pseudouridine modifications.突变型组氨酸转运RNA在抑制方面无效且缺乏两个假尿苷修饰。
Nat New Biol. 1972 Jul 19;238(81):72-4. doi: 10.1038/newbio238072a0.
9
Pleiotropy of hisT mutants blocked in pseudouridine synthesis in tRNA: leucine and isoleucine-valine operons.在tRNA假尿苷合成中受阻的hisT突变体的多效性：亮氨酸和异亮氨酸 - 缬氨酸操纵子。
Proc Natl Acad Sci U S A. 1974 May;71(5):1857-61. doi: 10.1073/pnas.71.5.1857.
10
Proton nuclear magnetic resonance of minor nucleosides in yeast phenylalanine transfer ribonucleic acid. Conformational changes as a consequence of aminoacylation, removal of the Y base, and codon--anticodon interaction.酵母苯丙氨酸转移核糖核酸中稀有核苷的质子核磁共振。氨基酰化、Y碱基去除以及密码子-反密码子相互作用导致的构象变化。
Biochemistry. 1979 Jul 24;18(15):3189-99. doi: 10.1021/bi00582a001.

酵母苯丙氨酸转运核糖核酸中核酸C2位氘代及通过核Overhauser效应测定ψ31假尿苷构象的方法

Procedure for C2 deuteration of nucleic acids and determination of A psi 31 pseudouridine conformation by nuclear Overhauser effect in yeast tRNAPhe.

作者信息

Roy S, Papastavros M Z, Redfield A G

出版信息

Nucleic Acids Res. 1982 Dec 20;10(24):8341-9. doi: 10.1093/nar/10.24.8341.

DOI:10.1093/nar/10.24.8341
PMID:6761652
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC327090/
Abstract

Nuclear Overhauser effect (NOE) combined with semispecific deuteration provides a general strategy for identification of exchangeable protons in nucleic base pairs, and has been extended to NOEs involving purine C2 protons in tRNA. Deuterated tri-ethyl orthoformate was condensed with 5(4)-amino imidazole 4(5)-carboxamide to yield C2 deuterated hypoxanthine. C2 deuterated hypoxanthine was fed to a purine requiring mutant of yeast and C2 deuterated yeast tRNAPhe was isolated. This C2 deuterated tRNAPhe was used to identify A psi 31 and U8-A14. A psi 31 was found to be bonded through N1H. The utility of C2 deuteration in nucleic acid NMR is thus demonstrated.

摘要

核Overhauser效应（NOE）与半特异性氘代相结合，为鉴定核酸碱基对中可交换质子提供了一种通用策略，并且已扩展到涉及tRNA中嘌呤C2质子的NOE。用氘代原甲酸三乙酯与5(4)-氨基咪唑4(5)-甲酰胺缩合，得到C2氘代次黄嘌呤。将C2氘代次黄嘌呤喂给需要嘌呤的酵母突变体，分离得到C2氘代酵母苯丙氨酸tRNA。这种C2氘代苯丙氨酸tRNA用于鉴定Aψ31和U8-A14。发现Aψ31通过N1H键合。由此证明了C2氘代在核酸核磁共振中的实用性。