Reina J, Blanco I, Munar M
Virology Unit, University Hospital Son Dureta, Universitat Illes Balears (UIB), Palma de Mallorca, Spain.
Clin Diagn Virol. 1996 Oct;7(1):63-7. doi: 10.1016/s0928-0197(96)00249-8.
We present a prospective study of the correlation between the human cytomegalovirus (HCMV) quantitative antigenemia with monoclonal antibody to p72 protein (immediate-early antigen) and the number of infected cell foci detected in the shell-vial culture. A comparative study was made of the value of quantitative antigenemia (pp65 and p72) in 14 patients.
The average value of the pp65 antigenemia was 195 pp65-positive PMNLs per 10(5) PMNLs (range 10-1000) and that of the p72, 21 p72-positive PMNLs per 10(5) PMNLs (range 0-120) (P < 0.001). The p72 antigenemia value represented 10.7% of the pp65 value (range 4.4-70%). A statistical correlation was observed between the total number of infected cell foci detected in the shell-vial culture and the total number of p72-positive PMNLs (P < 0.001), but not with the number of pp65-positive PMNLs (P = 0.4). A study of the number of infected cell foci detected in the shell-vial per 100000 PMNLs inoculated showed a statistical correlation with the value of the p72 antigenemia (P < 0.001).
According to results, there seems to be a general population of PMNLs carrying viral particles which are defected by means of the pp65 monoclonal antibody, and a subpopulation carrying active and replicative viral particles which is detected with the p72 antibody. This last subpopulation would be responsible for the formation of infected cell foci in the shell-vial culture. However due to the technical difficulties presented by the routine performance of p72 antigenemia, we recommend the routine application of the quantitative shell-vial culture and the use of the number of infected cell foci x 100000 PMNLs inoculated as a parameter of replicative viral load for the diagnosis of infection and disease caused by HCMV.
我们开展了一项前瞻性研究，以探讨人巨细胞病毒（HCMV）针对p72蛋白（即刻早期抗原）的单克隆抗体定量抗原血症与空斑小瓶培养中检测到的感染细胞灶数量之间的相关性。对14例患者的定量抗原血症（pp65和p72）值进行了比较研究。
pp65抗原血症的平均值为每10⁵个中性粒细胞中有195个pp65阳性中性粒细胞（范围为10 - 1000），p72抗原血症的平均值为每10⁵个中性粒细胞中有21个p72阳性中性粒细胞（范围为0 - 120）（P < 0.001）。p72抗原血症值占pp65值的10.7%（范围为4.4% - 70%）。在空斑小瓶培养中检测到的感染细胞灶总数与p72阳性中性粒细胞总数之间存在统计学相关性（P < 0.001），但与pp65阳性中性粒细胞数量无相关性（P = 0.4）。对接种的每100000个中性粒细胞在空斑小瓶中检测到的感染细胞灶数量进行研究，发现其与p72抗原血症值存在统计学相关性（P < 0.001）。
根据研究结果，似乎存在一部分携带病毒颗粒的中性粒细胞群体，这些病毒颗粒可通过pp65单克隆抗体检测到，还有一部分携带活跃复制病毒颗粒的亚群，可通过p72抗体检测到。最后这个亚群可能是空斑小瓶培养中感染细胞灶形成的原因。然而，由于p72抗原血症常规检测存在技术困难，我们建议常规应用定量空斑小瓶培养，并将接种的每100000个中性粒细胞中感染细胞灶的数量作为复制病毒载量的参数，用于诊断HCMV感染和相关疾病。
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