来自分化胚胎干细胞的染色体外环状DNA的分子特征分析

Molecular characterization of extrachromosomal circular DNAs from differentiating embryonic stem cells.

作者信息

Arakawa H, Shimizu T, Iwakura Y, Yamagishi H

机构信息

Department of Biophysics, Graduate School of Science, Kyoto University, Japan.

出版信息

Cell Struct Funct. 1996 Dec;21(6):451-7. doi: 10.1247/csf.21.451.

DOI:10.1247/csf.21.451

PMID:9078402

Abstract

Embryonic E14 stem cells were differentiated to parietal yolksac-like flat cells in vitro in the absence of added feeders and LIF (Leukemia Inhibitory Factor). We cloned circular DNAs from the differentiating E14 cells. Out of 9 DNA inserts with the unique sequence, one clone showed a chromosomal rearrangement which could have occurred between a pair of short inverted repeats. Recombination mechanism is discussed in view of two other circularization events of the flanking sequences between short inverted repeats shown in differentiated P19 embryonal carcinoma cells.

摘要

在不添加饲养层细胞和白血病抑制因子（LIF）的情况下，将胚胎E14干细胞在体外分化为卵黄囊样扁平细胞。我们从正在分化的E14细胞中克隆了环状DNA。在9个具有独特序列的DNA插入片段中，有一个克隆显示出一种染色体重排，这种重排可能发生在一对短反向重复序列之间。鉴于在分化的P19胚胎癌细胞中显示的短反向重复序列之间侧翼序列的另外两个环化事件，对重组机制进行了讨论。