HeLa细胞染色体外环状DNA中的L1序列：同源重组介导环化的证据
L1 sequences in HeLa extrachromosomal circular DNA: evidence for circularization by homologous recombination.
作者信息
Jones R S, Potter S S
出版信息
Proc Natl Acad Sci U S A. 1985 Apr;82(7):1989-93. doi: 10.1073/pnas.82.7.1989.
Abstract
Subcloned probes of the L1 family of repetitive elements were used to isolate L1-carrying clones from a plasmid library of HeLa cell extrachromosomal circular DNA. One clone was analyzed in detail by restriction mapping, cross-hybridization to L1 probes, and base sequence analysis. In addition to approximately the 3' half of a full-sized L1 element, this clone carried 390 base pairs of non-L1 sequence that is single copy in the HeLa genome. A HeLa genomic clone of this unique chromosomal region was isolated and the sequence organization of the circle clone was compared with the linear chromosomal region from which it was ultimately derived. We discuss possible mechanisms of circular DNA formation and propose homologous intrachromosomal recombination between 9-base-pair direct repeats to be most likely in this case.
摘要
重复元件L1家族的亚克隆探针被用于从HeLa细胞染色体外环状DNA的质粒文库中分离携带L1的克隆。通过限制性图谱分析、与L1探针的交叉杂交以及碱基序列分析，对其中一个克隆进行了详细研究。除了一个全长L1元件的大约3' 端外，该克隆还携带了390个碱基对的非L1序列，该序列在HeLa基因组中是单拷贝的。分离出了这个独特染色体区域的HeLa基因组克隆，并将环状克隆的序列组织与其最终来源的线性染色体区域进行了比较。我们讨论了环状DNA形成的可能机制，并提出在这种情况下，9个碱基对的直接重复序列之间的同源染色体内重组最有可能发生。
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