Rastawicki W, Räty R, Kleemola M
Department of Bacteriology, National Institute of Hygiene, Warsaw, Poland.
Diagn Microbiol Infect Dis. 1996 Nov-Dec;26(3-4):141-3. doi: 10.1016/s0732-8893(96)00216-7.
Paired serum specimens from 17 patients with Mycoplasma pneumoniae infection, as demonstrated by a rise in complement fixation (CF) antibody titer and 14 single sera, negative in the CF test, were tested for the presence of Ig A, Ig G, and Ig M antibodies to a polypeptide with a molecular weight of 170 KDa (P1) in an immunoblot assay. In acute phase sera, collected in the 1st week of the disease, frequency of occurrence of the antibodies against P1 protein did not exceed 18%. Two to three weeks later, Ig A antibodies were detectable in 82.4%, Ig M in 76.5%, and Ig G antibodies in all convalescent phase sera. No sera negative in the CF test (titer < 4) had antibodies against M. pneumoniae adhesin P1.
对17例肺炎支原体感染患者的配对血清标本（通过补体结合（CF）抗体滴度升高得以证实）以及14份CF试验阴性的单份血清，采用免疫印迹法检测针对分子量为170 kDa的多肽（P1）的IgA、IgG和IgM抗体。在疾病第1周采集的急性期血清中，针对P1蛋白的抗体出现频率不超过18%。两到三周后，在所有恢复期血清中，82.4%可检测到IgA抗体，76.5%可检测到IgM抗体，所有血清均可检测到IgG抗体。CF试验阴性（滴度<4）的血清中没有抗肺炎支原体黏附素P1的抗体。
