Fischer G S, Sweimler W I, Kleger B
Diagn Microbiol Infect Dis. 1986 Feb;4(2):139-45. doi: 10.1016/0732-8893(86)90148-3.
The MYCOPLASMELISA test kit was compared with the complement fixation test for detection of Mycoplasma pneumoniae antibody in single sera and evaluation of acute- and convalescent-phase serum pairs for significant rises in antibody titers. Agreement between the two assays was 96.6% (85 of 88) for antibody detection and 93.9% (92 of 98) for evaluation of paired sera. Sensitivity and specificity of MYCOPLASMELISA, relative to complement fixation, for the serodiagnosis of recent M. pneumoniae infection was 92.2% (59 of 64) and 95% (19 of 20), respectively.
将支原体酶联免疫吸附测定(MYCOPLASMELISA)检测试剂盒与补体结合试验进行比较,以检测单份血清中的肺炎支原体抗体,并评估急性期和恢复期血清对中抗体滴度的显著升高情况。两种检测方法在抗体检测方面的一致性为96.6%(88份中的85份),在评估配对血清方面的一致性为93.9%(98份中的92份)。相对于补体结合试验,MYCOPLASMELISA对近期肺炎支原体感染进行血清学诊断的敏感性和特异性分别为92.2%(64份中的59份)和95%(20份中的19份)。