通过酶联免疫吸附测定法分析非典型肺炎患者对肺炎支原体P1和P116的免疫反应。
Immune response to Mycoplasma pneumoniae P1 and P116 in patients with atypical pneumonia analyzed by ELISA.
作者信息
Drasbek Mette, Nielsen Pernille K, Persson Kenneth, Birkelund Svend, Christiansen Gunna
机构信息
Loke Diagnostics ApS, Forskerparken Aarhus, Gustav Wieds Vej 10 C, DK-8000 Aarhus C, Denmark.
出版信息
BMC Microbiol. 2004 Feb 5;4:7. doi: 10.1186/1471-2180-4-7.
BACKGROUND
Serology is often used for the diagnosis of Mycoplasma pneumoniae. It is important to identify specific antigens that can distinguish between the presence or absence of antibodies against M. pneumoniae. The two proteins, P116 and P1, are found to be immunogenic. By using these in ELISA it is possible to identify an immune response against M. pneumoniae in serum samples.
RESULTS
A recombinant protein derived from the P116 protein and one from the P1 protein were used in two ELISA tests, rP116-ELISA and rP1-ELISA. Human serum samples from patients with atypical pneumonia were tested and compared to the results of the complement fixation test. There was a good agreement between the two tests but the rP1-ELISA showed the best discrimination between positive and negative samples.
CONCLUSION
Two ELISA tests based on recombinant proteins have been analysed and compared to the complement fixation test results. The two ELISA tests were found suitable for use in serodiagnostics of M. pneumoniae infections. The use of specific antigens eliminates the risk of cross reaction to an immune response against other bacteria.
背景
血清学常用于肺炎支原体的诊断。识别能够区分抗肺炎支原体抗体存在与否的特异性抗原很重要。已发现两种蛋白质，即P116和P1具有免疫原性。通过在酶联免疫吸附测定（ELISA）中使用这些蛋白质，能够识别血清样本中针对肺炎支原体的免疫反应。
结果
源自P116蛋白的一种重组蛋白和源自P1蛋白的一种重组蛋白被用于两种ELISA检测，即重组P116-ELISA和重组P1-ELISA。对非典型肺炎患者的人血清样本进行检测，并与补体结合试验的结果进行比较。两种检测结果之间有良好的一致性，但重组P1-ELISA在阳性和阴性样本之间显示出最佳的区分度。
结论
已对基于重组蛋白的两种ELISA检测进行分析，并与补体结合试验结果进行比较。发现这两种ELISA检测适用于肺炎支原体感染的血清学诊断。使用特异性抗原消除了对其他细菌免疫反应发生交叉反应的风险。
Zotero 插件