Williams E L, Kleiboeker S B, Reddy P G, Clouser D F, Lehmkuhl H D
Department of Microbiology, School of Veterinary Medicine, Tuskegee University, Ala, USA.
Intervirology. 1996;39(4):275-9. doi: 10.1159/000150529.
Restriction endonuclease site maps were constructed for the genome of a caprine adenovirus (GAdV), strain NC90-7261, which was isolated in 1990 from a 3-year-old goat with encephalitis. Genomic GAdV DNA was digested with seven restriction endonucleases (RE). Genomic DNA libraries of GAdV were constructed by cloning BamHI and HindIII restriction fragments into a plasmid vector. Using cloned GAdV genomic fragments as probes in Southern blot hybridizations, an RE site map was constructed. The position of several clones was confirmed by limited nucleotide sequencing and the location of several RE sites was determined by single or double RE digestions of cloned fragments. The size of the GAdV genome was determined to be 28.2 kbp. The restriction pattern described in this report is different from that of other adenoviruses. Although the genomic organization of this GAdV is likely to be similar to that of other adenoviruses, the overall level of sequence similarity is low.
构建了一种山羊腺病毒（GAdV）NC90 - 7261株基因组的限制性内切酶位点图谱，该毒株于1990年从一只患有脑炎的3岁山羊中分离得到。用七种限制性内切酶（RE）消化基因组GAdV DNA。通过将BamHI和HindIII限制性片段克隆到质粒载体中构建了GAdV的基因组DNA文库。在Southern印迹杂交中使用克隆的GAdV基因组片段作为探针，构建了限制性内切酶位点图谱。通过有限的核苷酸测序确认了几个克隆的位置，并通过对克隆片段进行单酶切或双酶切确定了几个限制性内切酶位点的位置。确定GAdV基因组大小为28.2 kbp。本报告中描述的限制性图谱与其他腺病毒的不同。尽管这种GAdV的基因组组织可能与其他腺病毒相似，但序列相似性的总体水平较低。
