Ong-awyooth L, Ong-ajyooth S, Tiensong K, Nilwarangkur S
Department of Medicine, Faculty of Medicine, Siriraj Hospital, Mahidol University, Bangkok, Thailand.
J Med Assoc Thai. 1997 Feb;80(2):101-8.
Endogenous oxygen radical scavengers such as glutathione peroxidase (GSH-Px), catalase (CAT), glutathione and vitamin E are powerful regulatory systems against free radical toxicity. These oxidative injuries are increased in patients with chronic renal failure leading to various abnormalities including anemia. In this study, activities of GSH-Px, CAT, glutathione and vitamin E were measured in the erythrocytes of 54 chronic renal failure patients compared with 32 healthy controls. GSH-Px activities were lower significantly from controls (20.5 +/- 6.79 vs 28.3 +/- 9.0 u/gHb, p < 0.001). Erythrocytes CAT (6.52 +/- 2.3 vs 7.54 +/- 1.9 u/gHb, p < 0.05), glutathione (63.59 +/- 20.2 vs 75.1 +/- 6.3 mg/dl, p < 0.05) vit. E (2.23 +/- 0.53 vs 3.38 +/- 0.44 g/ml RBC, p < 0.001) were also lower in the patients group. Plasma malondialdehyde (MDA) known as lipid peroxidation product was higher significantly than controls (p < 0.001). Abnormal erythrocyte osmotic fragility test, expressed by glycerol lysis time (GLT50) was found in the patients group (p < 0.001) and correlated significantly with RBC vitamin E. Results demonstrated defects in erythrocytes enzymatic antioxidant defense mechanism in chronic renal failure patients. To improve antioxidant systems seems to be promising in preventing hemolysis and anemia in these patients.
内源性氧自由基清除剂，如谷胱甘肽过氧化物酶（GSH-Px）、过氧化氢酶（CAT）、谷胱甘肽和维生素E，是对抗自由基毒性的强大调节系统。慢性肾功能衰竭患者的这些氧化损伤会增加，导致包括贫血在内的各种异常。在本研究中，测量了54例慢性肾功能衰竭患者红细胞中GSH-Px、CAT、谷胱甘肽和维生素E的活性，并与32例健康对照者进行比较。GSH-Px活性显著低于对照组（20.5±6.79 vs 28.3±9.0 u/gHb，p<0.001）。患者组红细胞CAT（6.52±2.3 vs 7.54±1.9 u/gHb，p<0.05）、谷胱甘肽（63.59±20.2 vs 75.1±6.3 mg/dl，p<0.05）、维生素E（2.23±0.53 vs 3.38±0.44 g/ml RBC，p<0.001）也较低。作为脂质过氧化产物的血浆丙二醛（MDA）显著高于对照组（p<0.001）。患者组甘油溶解时间（GLT50）表示的红细胞渗透脆性试验异常（p<0.001），且与红细胞维生素E显著相关。结果表明慢性肾功能衰竭患者红细胞酶抗氧化防御机制存在缺陷。改善抗氧化系统似乎有望预防这些患者的溶血和贫血。
