Bongso T A, Basrur P K
Vet Rec. 1975 Feb 8;96(6):124-6. doi: 10.1136/vr.96.6.124.
A method for the aspiration of amniotic fluid from pregnant cows at 70 to 1000 day gestation and the subsequent cultivation of amniotic cells in vitro is described. The unit for aspirating amniotic fluid includes a sterile Plexitron tubing fitted with a 60 ml syringe on one end and a 12 in needle ("18 gauge) partially encased in a 6 inch AI pipette on the other. Aspiration of fluid is accomplished through the vaginal route by piercing the dorsal fornix and chromosome analysis is performed on amniotic cells after cultivation in vitro for four to seven days. This method, which is simple and accurate, provides a safe diagnostic procedure for the prenatal detection of sex and cytogenetic defects in cattle.
本文描述了一种在妊娠70至1000天的怀孕母牛身上抽取羊水，并随后在体外培养羊膜细胞的方法。羊水抽吸装置包括一根无菌的Plexitron管，一端装有一个60毫升的注射器，另一端是一根12英寸（18号）的针头，部分包裹在一个6英寸的人工授精移液管中。通过穿刺阴道穹窿经阴道途径完成液体抽吸，体外培养四至七天后对羊膜细胞进行染色体分析。这种方法简单准确，为牛的产前性别检测和细胞遗传学缺陷提供了一种安全的诊断程序。
