Saito H, Papaconstantinou J, Sato H, Goldstein S
Department of Human Biological Chemistry and Genetics, University of Texas Medical Branch, Galveston, Texas 77555-0643, USA.
J Biol Chem. 1997 Mar 28;272(13):8157-60. doi: 10.1074/jbc.272.13.8157.
We report here a novel cDNA clone with a predicted protein sequence similar to lysyl oxidase. This full-length cDNA clone of 3432 base pairs (WS9-14) was isolated from human fibroblasts on the basis of its overexpression in senescent cells. It encodes an 87-kDa polypeptide, whose protein is a member of the scavenger receptor cysteine-rich family, because it contains four scavenger receptor cysteine-rich domains that are found in several secreted or cell surface proteins. The WS9-14 protein has a 48% identity with both lysyl oxidase and lysyl oxidase-like protein at a region corresponding to exons 2-6, implying the existence of a lysyl oxidase gene family. The pattern of WS9-14 gene expression by fibroblasts parallels pro-collagen I-alpha1 expression. Its mRNA level is induced by transforming growth factor beta-1 and indomethacin and inhibited by phorbol ester and retinoic acid. WS9-14 is abundantly expressed in all tumor cell lines examined that attach to culture dishes but not in cell lines that grow in suspension and is also up-regulated in senescent fibroblasts. These results suggest that WS9-14 gene encodes an extracellular protein that may be specifically involved in cell adhesion and senescence.
我们在此报告一个新的cDNA克隆，其预测的蛋白质序列与赖氨酰氧化酶相似。这个3432个碱基对的全长cDNA克隆（WS9 - 14）是从人成纤维细胞中分离出来的，基于其在衰老细胞中的过表达。它编码一个87 kDa的多肽，其蛋白质是富含半胱氨酸的清道夫受体家族的成员，因为它包含在几种分泌蛋白或细胞表面蛋白中发现的四个富含半胱氨酸的清道夫受体结构域。WS9 - 14蛋白在对应于外显子2 - 6的区域与赖氨酰氧化酶和类赖氨酰氧化酶蛋白均有48%的同源性，这意味着存在一个赖氨酰氧化酶基因家族。成纤维细胞中WS9 - 14基因的表达模式与前胶原I - α1的表达相似。其mRNA水平受转化生长因子β - 1和吲哚美辛诱导，受佛波酯和视黄酸抑制。WS9 - 14在所有贴附于培养皿的检测肿瘤细胞系中大量表达，但在悬浮生长的细胞系中不表达，并且在衰老的成纤维细胞中也上调。这些结果表明WS9 - 14基因编码一种细胞外蛋白，可能特异性参与细胞黏附和衰老过程。
