Hu W S, Aunins J G
Chemical Engineering and Materials Science Department, University of Minnesota, 421 Washington Avenue South East, Minneapolis, MN 55455, USA.
Curr Opin Biotechnol. 1997 Apr;8(2):148-53. doi: 10.1016/s0958-1669(97)80093-6.
Mammalian cell culture continues to draw major research efforts. A great deal of progress has recently been made in cellular physiology, especially in factors adversely affecting cell growth or viability. Through molecular genetic manipulation, cells are more readily cultivated in a medium free of animal proteins. Achieving a high cell concentration and high viability continue to be common themes in engineering research. The need to implement a control policy for fed-batch and perfusion cultures has prompted increased efforts in process monitoring and control. Integrating these advances will be beneficial for ensuring product quality and process consistency.
哺乳动物细胞培养仍然吸引着大量的研究工作。最近在细胞生理学方面取得了很大进展，特别是在对细胞生长或活力有不利影响的因素方面。通过分子遗传操作，细胞更容易在无动物蛋白的培养基中培养。实现高细胞浓度和高活力仍然是工程研究中的常见主题。对分批补料和灌注培养实施控制策略的需求促使人们在过程监测和控制方面加大了努力。整合这些进展将有助于确保产品质量和过程一致性。
