de Boer E C, Somogyi L, de Ruiter G J, de Reijke T M, Kurth K H, Schamhart D H
Department of Urology, University of Amsterdam, The Netherlands.
Urol Res. 1997;25(1):31-4. doi: 10.1007/BF00941903.
In intravesical therapy for superficial bladder carcinoma urothelial cells may, through the production of cytokines, contribute to the bacillus Calmette-Guerin (BCG)-induced local immunological reaction and associated antitumor efficacy. The aim of this study was to investigate such a role for the neutrophil-attracting cytokine interleukin-8 (IL-8). The appearance of IL-8 in patients urine after BCG therapy was compared with BCG-induced IL-6 and IL-2 and the stability of IL-8 in urine was tested. Compared to IL-6 and IL-2, a rapid induction of IL-8 was observed, occurring after the first BCG instillation. Urinary IL-8 was highly stable, even after 24 h incubation at 37 degrees C. The IL-8 concentration after the first instillation seemed to be associated with subsequent development of an immune response. Consequently, IL-8 seems an attractive candidate for investigation of its prognostic value for a clinical response to BCG therapy.
在浅表性膀胱癌的膀胱内治疗中，尿路上皮细胞可能通过产生细胞因子，促进卡介苗（BCG）诱导的局部免疫反应及相关抗肿瘤疗效。本研究旨在探讨趋化中性粒细胞的细胞因子白细胞介素-8（IL-8）的这一作用。将BCG治疗后患者尿液中IL-8的出现情况与BCG诱导产生的IL-6和IL-2进行比较，并检测IL-8在尿液中的稳定性。与IL-6和IL-2相比，观察到IL-8在首次BCG灌注后迅速诱导产生。尿IL-8非常稳定，即使在37℃孵育24小时后也是如此。首次灌注后的IL-8浓度似乎与随后免疫反应的发展有关。因此，IL-8似乎是一个有吸引力的研究对象，可用于探讨其对BCG治疗临床反应的预后价值。
