Maas R, Wang C
Department of Microbiology, New York University Medical Center, New York 10016, USA.
J Bacteriol. 1997 Apr;179(7):2163-8. doi: 10.1128/jb.179.7.2163-2168.1997.
Using a sensitive primer extension technique, we have carried out studies to localize the start site of replication of the replicon RepFIC. In the course of these studies, we have found evidence that supports the hypothesis that transcription is an integral component of the initiation of replication. On the basis of our findings, we suggest that the transcript is processed to act as a primer, and therefore we propose that the transcript has a dual role as primer of replication and mRNA for the RepA1 protein. We present a model, based on our evidence, for the initiation of replication of the replicon RepFIC. This model provides as well an alternative explanation for what has been called the cis action of RepA1, and we show that RepA1 may act in trans as well as in cis.
我们使用一种灵敏的引物延伸技术开展研究，以定位复制子RepFIC的复制起始位点。在这些研究过程中，我们发现了支持转录是复制起始不可或缺组成部分这一假说的证据。基于我们的发现，我们认为转录本被加工后充当引物，因此我们提出转录本具有作为复制引物和RepA1蛋白的信使核糖核酸（mRNA）的双重作用。我们基于证据提出了一个复制子RepFIC复制起始的模型。该模型也为所谓的RepA1顺式作用提供了另一种解释，并且我们表明RepA1可能以反式和顺式发挥作用。
