肠毒素质粒EntP307的RepFIC复制子的复制控制蛋白RepA1的结晶及初步晶体学分析

Crystallization and preliminary crystallographic analysis of RepA1, a replication control protein of the RepFIC replicon of enterotoxin plasmid EntP307.

作者信息

Song H, Phillips S E, Parsons M R, Maas R

机构信息

Department of Biochemistry and Molecular Biology, University of Leeds, United Kingdom.

出版信息

Proteins. 1996 May;25(1):137-8. doi: 10.1002/(SICI)1097-0134(199605)25:1<137::AID-PROT13>3.0.CO;2-L.

DOI:10.1002/(SICI)1097-0134(199605)25:1<137::AID-PROT13>3.0.CO;2-L

PMID:8727327

Abstract

RepA1 protein is essential for replication of the RepFIC replicon of enterotoxin plasmid EntP307 and is thought to interact directly with the origin of replication. We have purified RepA1 from an over-producing expression system and have prepared single crystals using a macroseeding technique. The crystals belong to space group P2(1)2(1)2(1) or P2(1)2(1)2, with cell dimensions a = 61 A, b = 67 A, and c = 243 A. They diffract X-rays to 3.3 A resolution and probably contain two 40,000 molecular weight RepA1 molecules per asymmetric unit.

摘要

RepA1蛋白对于肠毒素质粒EntP307的RepFIC复制子的复制至关重要，并且被认为直接与复制起点相互作用。我们已经从一个过量表达系统中纯化了RepA1，并使用宏观接种技术制备了单晶。这些晶体属于空间群P2(1)2(1)2(1)或P2(1)2(1)2，晶胞参数为a = 61 Å，b = 67 Å，c = 243 Å。它们能将X射线衍射到3.3 Å的分辨率，并且每个不对称单元可能包含两个分子量为40,000的RepA1分子。