Paul B D, Mitchell J M, Burbage R, Moy M, Sroka R
Navy Drug Screening Laboratory, Norfolk, VA 23511-6295.
J Chromatogr. 1990 Jul 13;529(1):103-12. doi: 10.1016/s0378-4347(00)83811-6.
A sensitive method for the detection and quantitation of lysergic acid diethylamide (LSD) in urine was developed. After initial solvent extraction, the compound was further purified by liquid-liquid extraction or by solid-phase extraction. The trimethylsilyl derivative of LSD was detected by gas chromatography-mass spectrometry (GC-MS) operated in the electron-impact mode with selected-ion monitoring. The presence of LSD was confirmed by comparing retention times and relative abundances of ions of unknowns with that of a standard. The recovery of this procedure was greater than 89%. The intra-run and inter-run coefficients of variation were less than 5% and less than 7%, respectively. This procedure allows detection of LSD concentrations as low as 29 pg/ml. Quantitation of LSD was linear over the concentration range 50-2000 pg/ml.
开发了一种用于检测和定量尿液中麦角酸二乙酰胺（LSD）的灵敏方法。在初步溶剂萃取后，该化合物通过液-液萃取或固相萃取进一步纯化。LSD的三甲基硅烷基衍生物通过在电子轰击模式下运行并采用选择离子监测的气相色谱-质谱联用仪（GC-MS）进行检测。通过比较未知物与标准品的保留时间和离子相对丰度来确认LSD的存在。该方法的回收率大于89%。批内和批间变异系数分别小于5%和小于7%。此方法能够检测低至29 pg/ml的LSD浓度。LSD在50-2000 pg/ml的浓度范围内定量呈线性。
