Germadnik D, Pilger A, Rüdiger H W
Department of Occupational Medicine, University of Vienna/Vienna General Hospital, Austria.
J Chromatogr B Biomed Sci Appl. 1997 Feb 21;689(2):399-403. doi: 10.1016/s0378-4347(96)00328-3.
A high-performance liquid chromatographic procedure with electrochemical detection is described for the determination of urinary 8-hydroxy-2'-deoxyguanosine, a major oxidative DNA lesion induced by radical forming agents. A two-step solid-phase extraction procedure was followed for extracting 8-hydroxy-2'-deoxyguanosine from human urine and the analysis was performed on a RP-18 analytical column under isocratic conditions. The limit of detection of 8-hydroxy-2'-deoxyguanosine in urine was found to be 0.9 nM. The non-invasive assay provides an indirect measurement of oxidative DNA damage.
描述了一种采用电化学检测的高效液相色谱法，用于测定尿中的8-羟基-2'-脱氧鸟苷，这是一种由自由基形成剂诱导产生的主要氧化性DNA损伤产物。采用两步固相萃取法从人尿中提取8-羟基-2'-脱氧鸟苷，并在等度条件下于RP - 18分析柱上进行分析。发现尿中8-羟基-2'-脱氧鸟苷的检测限为0.9 nM。这种非侵入性检测方法可间接测量氧化性DNA损伤。
