Huh Y, Kim C, Lee W, Kim J, Ahn H
Department of Anatomy, College of Medicine, Kyunghee University, Seoul, South Korea.
Neurosci Lett. 1997 Feb 28;223(3):157-60. doi: 10.1016/s0304-3940(97)13430-9.
Age-related changes of neuropeptide Y (NPY) and nicotinamide adenine dinucleotide phosphate-diaphorase (NADPH-d) were examined in the rat cerebral cortex and striatum by immunohistochemical and histochemical methods. Double labeling for NPY and NADPH-d showed that about 30-70% of NPY-immunoreactive (NPY-IR) neurons in the cerebral cortex of the control (4-month-old) rats contained NADPH-d and that 50-75% in the aged (24-month-old) rats. The aged rats showed a significant increase in percentage of colocalization of NPY and NADPH-d in comparison with the control rats in the temporal cortex, occipital cortex, cingulate cortex, insular cortex, retrosplenial cortex and caudatoputamen. However, colocalization percentage between control and aged rats in the frontal cortex, parietal cortex, perirhinal cortex, entorhinal cortex and nucleus accumbens were practically identical. In the aged group, the number of NPY-IR/NADPH-d-positive neurons was not significantly decreased in the cerebral cortex and striatum compared to the control group. However, the number of NPY-IR/NADPH-d-negative neurons was significantly decreased in all cerebral cortical areas and caudatoputamen in the aged group except in the nucleus accumbens. Major loss of NPY-IR/NADPH-d-negative neurons in the aged group were observed in the neurons of layer II/III and V/VI. These results demonstrate that the NADPH-d containing NPY-IR neurons are less influenced by aging than the control group in the cerebral cortex and striatum of rats.
采用免疫组织化学和组织化学方法，研究了大鼠大脑皮质和纹状体中神经肽Y（NPY）和烟酰胺腺嘌呤二核苷酸磷酸黄递酶（NADPH-d）的年龄相关变化。NPY和NADPH-d的双重标记显示，对照（4月龄）大鼠大脑皮质中约30%-70%的NPY免疫反应性（NPY-IR）神经元含有NADPH-d，而老年（24月龄）大鼠中这一比例为50%-75%。与对照大鼠相比，老年大鼠在颞叶皮质、枕叶皮质、扣带皮质、岛叶皮质、压后皮质和尾壳核中NPY与NADPH-d的共定位百分比显著增加。然而，对照大鼠和老年大鼠在额叶皮质、顶叶皮质、梨状周皮质、内嗅皮质和伏隔核中的共定位百分比实际上是相同的。在老年组中，与对照组相比，大脑皮质和纹状体中NPY-IR/NADPH-d阳性神经元的数量没有显著减少。然而，除伏隔核外，老年组所有大脑皮质区域和尾壳核中NPY-IR/NADPH-d阴性神经元的数量均显著减少。在老年组中，NPY-IR/NADPH-d阴性神经元的主要丢失发生在II/III层和V/VI层的神经元中。这些结果表明，在大鼠大脑皮质和纹状体中，含NADPH-d的NPY-IR神经元受衰老的影响小于对照组。
