血液和尿液中L(+)丙氨酸定量测定的快速酶促微量法。

Rapid enzymic micromethod for the quantitative determination of L(+)alanine in blood and urine.

作者信息

Schutgens R B, Awie C T, Beemer F A, Berntssen W J

出版信息

Clin Chim Acta. 1977 Oct 1;80(1):1-5. doi: 10.1016/0009-8981(77)90257-1.

Abstract

A micromethod for the quantitative determination of L-alanine in blood and urine is described. No interference of D-alanine, D-cysteine, L-proline, L-glutamine, L-valine, L-histidine or glycine in concentrations up to 5 mmol/l was found. Using this method fasting blood levels of L-alanine in children were determined. Defects in gluconeogenesis can be easily established by measuring with this method the in vivo L-alanine metabolism in an oral, or intravenous L-alanine loading test.

摘要

本文描述了一种定量测定血液和尿液中L-丙氨酸的微量方法。未发现浓度高达5 mmol/l的D-丙氨酸、D-半胱氨酸、L-脯氨酸、L-谷氨酰胺、L-缬氨酸、L-组氨酸或甘氨酸有干扰。使用该方法测定了儿童空腹血液中的L-丙氨酸水平。通过在口服或静脉注射L-丙氨酸负荷试验中用该方法测量体内L-丙氨酸代谢，可轻松确定糖异生缺陷。