Suvanto P, Hiltunen J O, Arumäe U, Moshnyakov M, Sariola H, Sainio K, Saarma M
Institute of Biotechnology, Viikinkaari 9, University of Helsinki, Helsinki, Finland.
Eur J Neurosci. 1996 Apr;8(4):816-22. doi: 10.1111/j.1460-9568.1996.tb01267.x.
The localization of glial cell line-derived neurotrophic factor (GDNF) mRNA was studied by in situ hybridization in rat from embryonic (E) day E10 to E15. At E10, GDNF mRNA is found in the urogenital field and the cranial part of the gut. At E11, the most abundant expression of GDNF mRNA is seen in the epithelial cells of the second, third and fourth pharyngeal pouches, the third and fourth pharyngeal arches and pharynx. Also mesenchymal cells of the gut and mesonephric tubules contain GDNF mRNA. At E13, expression is observed in the mesenchymal cell layers of the oesophagus, intestine and stomach, the mesenchymal cells around the condensing cartilages and metanephric kidney mesenchyme. Also, the epithelia of Rathke's pouch and pharynx are intensely labelled. High expression of GDNF mRNA continues at E15 in kidney, gastrointestinal tract and cartilage. At that stage, GDNF mRNA is seen also in whisker pad and skeletal muscles. The distribution of GDNF mRNA in embryonic rat suggests important roles for GDNF in the early differentiation of the kidney tubules, the innervation of the gastrointestinal tract and the differentiation process of the cartilage and muscle. Our results indicate novel functions for GDNF outside the nervous system.
通过原位杂交技术，研究了从胚胎（E）第10天到第15天大鼠体内胶质细胞系源性神经营养因子（GDNF）mRNA的定位情况。在E10时，GDNF mRNA存在于泌尿生殖区域和肠道的头端部分。在E11时，GDNF mRNA在第二、第三和第四咽囊、第三和第四咽弓以及咽部的上皮细胞中表达最为丰富。肠道的间充质细胞和中肾小管也含有GDNF mRNA。在E13时，在食管、肠道和胃的间充质细胞层、正在凝聚的软骨周围的间充质细胞以及后肾间充质中观察到表达。此外，拉特克囊和咽部的上皮也被强烈标记。在E15时，GDNF mRNA在肾脏、胃肠道和软骨中持续高表达。在那个阶段，在触须垫和骨骼肌中也能看到GDNF mRNA。GDNF mRNA在胚胎大鼠中的分布表明，GDNF在肾小管的早期分化、胃肠道的神经支配以及软骨和肌肉的分化过程中发挥着重要作用。我们的结果表明GDNF在神经系统之外具有新的功能。
