Utoh E, Okazaki J, Gonda Y
First Department of Prosthodontics, Osaka Dental University, Japan.
J Osaka Dent Univ. 1998 Apr;32(1):1-7.
We determined the hyaluronic acid disaccharides, delta Di-HA, in the gingival crevicular fluid (GCF) and whole saliva of patients with periodontal disease, and in the peri-implant sulcus fluid (PISF) from sites around titanium osseointegrated implants, and compared these values with those in the GCF and whole saliva of controls. We also determined values for chondroitin sulfate disaccharide isomers at the same time. Glycosaminoglycans were extracted by digestion with Pronase E, followed by digestion of GAGs with hyaluronidase SD and chondroitinase ACII. Unsaturated disaccharide isomers produced from hyaluronic acid and chondroitin sulfate were analyzed by high-performance liquid chromatography (HPLC). The hyaluronic acid disaccharide delta Di-HA was found in all samples of GCF, PISF and whole saliva. The concentration of delta Di-HA in both GCF and whole saliva of the periodontitis group was greater than that in the controls. There was no difference in the concentration of delta Di-HA between the PISF and GCF of the controls. The ratios of hyaluronic acid to chondroitin sulfate in the GCF and in the whole saliva of the periodontitis group were significantly lower than that of the controls. There was no difference between the ratios in PISF and those in GCF of the controls. These results indicate that checking hyaluronic acid in GCF and whole saliva using HPLC is a useful means of assessing the condition of periodontal tissues, and that assaying hyaluronic acid in PISF may also be effective for monitoring the condition of tissues around dental implants.
我们测定了牙周病患者龈沟液（GCF）和全唾液中以及钛骨整合种植体周围部位的种植体周沟液（PISF）中的透明质酸二糖（δDi - HA），并将这些值与对照组GCF和全唾液中的值进行比较。我们同时还测定了硫酸软骨素二糖异构体的值。通过用链霉蛋白酶E消化来提取糖胺聚糖，随后用透明质酸酶SD和软骨素酶ACII消化糖胺聚糖。由透明质酸和硫酸软骨素产生的不饱和二糖异构体通过高效液相色谱（HPLC）进行分析。在所有GCF、PISF和全唾液样本中均发现了透明质酸二糖δDi - HA。牙周炎组GCF和全唾液中δDi - HA的浓度均高于对照组。对照组PISF和GCF中δDi - HA的浓度无差异。牙周炎组GCF和全唾液中透明质酸与硫酸软骨素的比值显著低于对照组。对照组PISF中的比值与GCF中的比值无差异。这些结果表明，使用HPLC检测GCF和全唾液中的透明质酸是评估牙周组织状况的一种有用方法，并且检测PISF中的透明质酸对于监测牙种植体周围组织的状况可能也有效。
