Merrell P, Carpenter W, Silverman S, Leider A, Gallo J
Department of Diagnostic Sciences, School of Dentistry, University of the Pacific, San Francisco, Calif., USA.
Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 1997 Mar;83(3):367-72. doi: 10.1016/s1079-2104(97)90245-6.
Monoclonal antibodies (MAbs) 17.13 and 63.12 exhibit characteristic reactivity patterns in normal stratified squamous epithelium, as well as highly sensitive and specific altered reactivity patterns in squamous cell carcinoma. The purpose of this study was to critically evaluate the patterns of reactivity of MAbs 17.13 and 63.12 in 43 biopsies of clinical oral leukoplakia or erythroleukoplakia with microscopic diagnoses of hyperkeratosis or epithelial dysplasia. Altered carcinoma-like reactivity patterns were seen in 72% of hyperkeratoses and in all cases of epithelial dysplasia, but varied in the level of epithelial strata exhibiting altered reactivity. Increased frequency of altered reactivity within the epithelial strata was associated with the presence, but not the grade of, epithelial dysplasia, as well as with the presence, intensity; and pattern of submucosal inflammation. The results of this study suggest that altered reactivity patterns of MAb 17.13 are associated with epithelial dysplasia and may be of assistance in detecting precancerous changes in hyperkeratoses before morphologically identifiable epithelial dysplasia. The association of submucosal inflammation with altered MAbs 17.13 and 63.12 reactivity may indicate either a decrease in specificity of these antibodies for precancerous change or an increased significance of inflammation in precancerous lesions.
单克隆抗体（MAb）17.13和63.12在正常分层鳞状上皮中呈现出特征性反应模式，在鳞状细胞癌中则表现出高度敏感和特异的改变反应模式。本研究的目的是严格评估MAb 17.13和63.12在43例临床诊断为口腔白斑或红白斑且显微镜诊断为角化过度或上皮发育异常的活检组织中的反应模式。在72%的角化过度病例和所有上皮发育异常病例中均可见到类似癌的改变反应模式，但在出现改变反应的上皮层水平上有所不同。上皮层内改变反应的频率增加与上皮发育异常的存在有关，而非其分级，还与黏膜下炎症的存在、强度及模式有关。本研究结果表明，MAb 17.13的改变反应模式与上皮发育异常相关，可能有助于在形态学上可识别的上皮发育异常之前检测角化过度中的癌前变化。黏膜下炎症与MAb 17.13和63.12反应改变的关联可能表明这些抗体对癌前变化的特异性降低，或者炎症在癌前病变中的意义增加。
