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从双峰驼乳腺和血液中分离出的吞噬细胞的呼吸爆发活性。

Respiratory burst activity of phagocytic cells isolated from the mammary glands and blood of camels (Camelus bactrianus).

作者信息

Cooray R, Abdurahaman O A, Bornstein S, Holmberg O, Aström G

机构信息

Department of Mastitis, National Veterinary Institute, Uppsala, Sweden.

出版信息

Zentralbl Veterinarmed B. 1997 Mar;44(1):29-37. doi: 10.1111/j.1439-0450.1997.tb00947.x.

DOI:10.1111/j.1439-0450.1997.tb00947.x
PMID:9084231
Abstract

The oxidative burst activity of phagocytic cells isolated from camel blood and mammary glands was studied using a chemiluminescence (Cl) assay. The polymorphonuclear leucocytes (PMNL) isolated from camel blood mounted a luminol-dependent Cl response upon stimulation with opsonized zymosan or opsonized Staphylococcus aureus. These responses showed an overall similarity to those described in other mammalian species. The leucocytes isolated from the mammary glands mounted Cl responses similar to those observed with blood PMNL but to a lower magnitude. Like the Cl responses of blood cells, the Cl responses induced by mammary gland leucocytes were associated with degranulation and the release of lysosomal enzymes such as myeloperoxidase (MPO).

摘要

利用化学发光(Cl)分析法研究了从骆驼血液和乳腺中分离出的吞噬细胞的氧化爆发活性。从骆驼血液中分离出的多形核白细胞(PMNL)在用调理酵母聚糖或调理金黄色葡萄球菌刺激后产生了依赖鲁米诺的Cl反应。这些反应与其他哺乳动物物种中描述的反应总体相似。从乳腺中分离出的白细胞产生的Cl反应与血液中的PMNL观察到的反应相似,但强度较低。与血细胞的Cl反应一样,乳腺白细胞诱导的Cl反应与脱颗粒以及溶酶体酶如髓过氧化物酶(MPO)的释放有关。

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