甲酰甲硫氨酰-亮氨酰-苯丙氨酸诱导牛多形核白细胞的化学发光反应。
Formylmethionyl-leucyl-phenylalanine-induced chemiluminescence responses in bovine polymorphonuclear leukocytes.
作者信息
Cooray R
机构信息
National Veterinary Institute, Uppsala, Sweden.
出版信息
Comp Immunol Microbiol Infect Dis. 1996 Jan;19(1):1-8. doi: 10.1016/0147-9571(95)00025-9.
Isolated bovine polymorphonuclear leukocytes (PMNL) show a biphasic luminol-dependent chemiluminescence response, and when exposed to high concentrations (2 x 10(-4) M) of the chemotactic peptide formylmethionyl-leucyl-phenylalanine (f-Met-Leu-Phe), the second emission peak is enhanced. This response was increased in magnitude by incubating PMNL at room temperature before stimulation with f-Met-Leu-Phe. Pre-exposure of bovine PMNL to myeloperoxidase (MPO) also enhanced their receptiveness to the chemotactic peptide, indicating that the MPO-hydrogen peroxide system can modulate the bovine PMNL response to chemotactic factors. The results demonstrate that bovine PMNL are stimulated by the chemotactic peptides to participate in inflammatory processes.
分离出的牛多形核白细胞（PMNL）表现出双相鲁米诺依赖性化学发光反应，当暴露于高浓度（2×10⁻⁴ M）的趋化肽甲酰甲硫氨酰 - 亮氨酰 - 苯丙氨酸（f - Met - Leu - Phe）时，第二个发射峰增强。在用f - Met - Leu - Phe刺激之前，将PMNL在室温下孵育会使这种反应的幅度增加。牛PMNL预先暴露于髓过氧化物酶（MPO）也增强了它们对趋化肽的反应性，这表明MPO - 过氧化氢系统可以调节牛PMNL对趋化因子的反应。结果表明，趋化肽刺激牛PMNL参与炎症过程。
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