甲酰甲硫氨酰-亮氨酰-苯丙氨酸诱导牛多形核白细胞的化学发光反应。

Formylmethionyl-leucyl-phenylalanine-induced chemiluminescence responses in bovine polymorphonuclear leukocytes.

作者信息

Cooray R

机构信息

National Veterinary Institute, Uppsala, Sweden.

出版信息

Comp Immunol Microbiol Infect Dis. 1996 Jan;19(1):1-8. doi: 10.1016/0147-9571(95)00025-9.

DOI:10.1016/0147-9571(95)00025-9

PMID:8654040

Abstract

Isolated bovine polymorphonuclear leukocytes (PMNL) show a biphasic luminol-dependent chemiluminescence response, and when exposed to high concentrations (2 x 10(-4) M) of the chemotactic peptide formylmethionyl-leucyl-phenylalanine (f-Met-Leu-Phe), the second emission peak is enhanced. This response was increased in magnitude by incubating PMNL at room temperature before stimulation with f-Met-Leu-Phe. Pre-exposure of bovine PMNL to myeloperoxidase (MPO) also enhanced their receptiveness to the chemotactic peptide, indicating that the MPO-hydrogen peroxide system can modulate the bovine PMNL response to chemotactic factors. The results demonstrate that bovine PMNL are stimulated by the chemotactic peptides to participate in inflammatory processes.

摘要

分离出的牛多形核白细胞（PMNL）表现出双相鲁米诺依赖性化学发光反应，当暴露于高浓度（2×10⁻⁴ M）的趋化肽甲酰甲硫氨酰 - 亮氨酰 - 苯丙氨酸（f - Met - Leu - Phe）时，第二个发射峰增强。在用f - Met - Leu - Phe刺激之前，将PMNL在室温下孵育会使这种反应的幅度增加。牛PMNL预先暴露于髓过氧化物酶（MPO）也增强了它们对趋化肽的反应性，这表明MPO - 过氧化氢系统可以调节牛PMNL对趋化因子的反应。结果表明，趋化肽刺激牛PMNL参与炎症过程。