Arashiro D S, Rapley J W, Cobb C M, Killoy W J
Department of Periodontics, University of Missouri-Kansas City School of Dentistry 64108, USA.
Int J Periodontics Restorative Dent. 1996 Oct;16(5):479-91.
The purpose of this study was to histologically compare the healing of porcine skin incisions made by CO2 laser, electrosurgery, and conventional scalpel. Incisions were made on the dorsal thorax of two microswine at staggered time intervals allowing the harvest of specimens at 0, 4, 7, 14, and 21 days postsurgery at the time of sacrifice. Each of the following histologic zones were identified and measured for both laser and electrosurgery incisions: (1) zone of tissue ablation. (2) zone of thermal necrosis, and (3) width of surface damage. The depth of cut and width of surface opening was measured for the scalpel incision. Furthermore, for each specimen, the presence and character of inflammatory cell infiltrate was noted, as well as the relative times at which bridging of the incision by connective tissue and coverage of the wound by an intact epithelial layer had occurred. Results showed that in this wound-healing model, the scalpel incisions produced more defined borders, healed more rapidly, and resulted in less collateral tissue damage than those produced by CO2 laser or electrosurgery. It was also noted that to create a wound 1.29 mm in depth required at least five passes of the laser beam within the same line of incision using 206.4 J/cm2 (6 W, continuous mode).
本研究的目的是从组织学角度比较二氧化碳激光、电外科手术和传统手术刀造成的猪皮肤切口的愈合情况。在两只小型猪的背部胸部以交错的时间间隔进行切口，以便在牺牲时于术后0、4、7、14和21天采集标本。对激光和电外科手术切口的以下每个组织学区域进行识别和测量：（1）组织消融区。（2）热坏死区，以及（3）表面损伤宽度。测量手术刀切口的切割深度和表面开口宽度。此外，对每个标本记录炎性细胞浸润的存在和特征，以及结缔组织使切口桥接和完整上皮层覆盖伤口的相对时间。结果表明，在这个伤口愈合模型中，手术刀切口产生的边界更清晰，愈合更快，与二氧化碳激光或电外科手术造成的切口相比，附带组织损伤更少。还注意到，使用206.4 J/cm²（6 W，连续模式）在同一切口线上至少进行五次激光束照射才能形成深度为1.29 mm的伤口。
