Swain M, Brisson J R, Sprott G D, Cooper F P, Patel G B
Institute for Biological Sciences, National Research Council of Canada, Ottawa, Ont., Canada.
Biochim Biophys Acta. 1997 Mar 10;1345(1):56-64. doi: 10.1016/s0005-2760(96)00163-4.
The main polar lipid (MPL) of Thermoplasma acidophilum has been purified and its structure determined. NMR, mass spectrometry, and capillary gas chromatography-mass spectrometry experiments have shown that the previously unidentified sugar moiety of MPL is the rare sugar L-gulose. MPL is thus a tetraether lipid with cyclopentane rings and head groups of phosphoglycerol, as previously reported, and beta-L-gulopyranose. Further, MPL is also the dominant lipid found in lipid extracts from another species of the Thermoplasma genus, T. volcanium, suggesting that L-gulose may represent a dominant sugar moiety of the polar lipids biosynthesized by this archaeobacterial genus. Minor phospholipids were tentatively identified as diether and hydroxydiether analogs of phosphatidylglycerol, and phosphatidylinositol.
嗜酸热原体的主要极性脂质（MPL）已被纯化并确定其结构。核磁共振、质谱和毛细管气相色谱-质谱实验表明，MPL中先前未鉴定的糖部分是稀有糖L-古洛糖。因此，MPL是一种具有环戊烷环和磷酸甘油头部基团的四醚脂质，如先前报道的那样，还有β-L-吡喃古洛糖。此外，MPL也是在热原体属的另一个物种火山热原体的脂质提取物中发现的主要脂质，这表明L-古洛糖可能是这种古细菌属生物合成的极性脂质的主要糖部分。少量磷脂被初步鉴定为磷脂酰甘油、磷脂酰肌醇的二醚和羟基二醚类似物。
