首次从古菌 Sulfolobus 菌株中分离和结构阐明 GDNT-β-Glu - 四醚脂质片段。

First Isolation and Structure Elucidation of GDNT-β-Glu - Tetraether Lipid Fragment from Archaeal Sulfolobus Strains.

机构信息

Institute for Bioprocessing and Analytical Measurement Techniques, Rosenhof, 37308, Heilbad Heiligenstadt, Germany.

Department of Chemistry, Martin Luther University Halle-Wittenberg, Kurt-Mothes-Str. 2, 06120, Halle, Germany.

出版信息

ChemistryOpen. 2021 Sep;10(9):889-895. doi: 10.1002/open.202100154.

DOI:10.1002/open.202100154

PMID:34468091

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8409090/

Abstract

Due to their special chemical structure, tetraether lipids (TEL) represent essential elements of archaeal membranes, providing these organisms with extraordinary properties. Here we describe the characterization of a newly isolated structural element of the main lipids. The TEL fragment GDNT-β-Glu was isolated from Sulfolobus metallicus and characterized in terms of its chemical structure by NMR- and MS-investigations. The obtained data are dissimilar to analogically derived established structures - in essence, the binding relationships in the polar head group are re-determined and verified. With this work, we provide an important contribution to the structure elucidation of intact TEL also contained in other Sulfolobus strains such as Solfulobus acidocaldarius and Sulfolobus solfataricus.

摘要

由于其特殊的化学结构，四醚脂质（TEL）是古菌膜的基本组成部分，赋予这些生物特殊的性质。在这里，我们描述了一种新分离的主要脂质结构元素的特征。从 Sulfolobus metallicus 中分离出 TEL 片段 GDNT-β-Glu，并通过 NMR 和 MS 研究对其化学结构进行了表征。得到的数据与类似衍生的已建立结构不同——本质上，重新确定和验证了极性头基团中的结合关系。通过这项工作，我们为阐明完整的 TEL 结构做出了重要贡献，这些 TEL 也存在于其他 Sulfolobus 菌株中，如 Solfulobus acidocaldarius 和 Sulfolobus solfataricus。