Hasséus B, Wallström M, Osterdahl B G, Hirsch J M, Jontell M
Department of Endodontology/Oral Diagnosis, Faculty of Odontology, Göteborg University, Sweden.
Eur J Oral Sci. 1997 Feb;105(1):45-51. doi: 10.1111/j.1600-0722.1997.tb00179.x.
Smokeless tobacco (ST) is known to adversely effect the oral mucosa, but knowledge about the influence on immune defence is limited. Few studies have investigated the effect of ST on the local immune response. In the present study, we have assessed the effect of a crude Swedish moist snuff (SS) extract, alkaloids, and nitrosamines on T-cell mitogenic response to Con A using epithelial cells, including Langerhans cells, of the rat oral mucosa as accessory cells. SS extract at a concentration of 4% reduced the T-cell proliferation by 50% (IC50 = 4%). Pretreatment of either oral epithelial cells or T-cells with SS extract also gave a significant inhibition of T-cell proliferation. This effect was not obtained following preincubation with SS components as alkaloids and different tobacco-specific nitrosamines (TSNA). None of the tested compounds were found to possess any mitogenic properties. This in vitro study showed that SS extract can evoke an immunosuppressive effect on mitogen-driven T-cell proliferation using cells from oral epithelium as accessory cells. This effect was more pronounced when SS extract was employed compared to when the single SS components were used alone.
已知无烟烟草（ST）会对口腔黏膜产生不良影响，但关于其对免疫防御影响的了解有限。很少有研究调查ST对局部免疫反应的影响。在本研究中，我们使用大鼠口腔黏膜的上皮细胞（包括朗格汉斯细胞）作为辅助细胞，评估了一种瑞典粗制湿鼻烟（SS）提取物、生物碱和亚硝胺对T细胞对刀豆蛋白A促有丝分裂反应的影响。浓度为4%的SS提取物使T细胞增殖降低了50%（半数抑制浓度=4%）。用SS提取物预处理口腔上皮细胞或T细胞也能显著抑制T细胞增殖。用生物碱和不同的烟草特异性亚硝胺（TSNA）等SS成分预孵育后未获得这种效果。未发现任何测试化合物具有促有丝分裂特性。这项体外研究表明，SS提取物可以利用口腔上皮细胞作为辅助细胞，对有丝分裂原驱动的T细胞增殖产生免疫抑制作用。与单独使用单一SS成分相比，使用SS提取物时这种效果更明显。
