Litwin C M, Leonard R B, Carroll K C, Drummond W K, Pavia A T
Department of Pathology, University of Utah, Salt Lake City 84132, USA.
J Infect Dis. 1997 Apr;175(4):864-70. doi: 10.1086/513983.
Shigellosis is hyperendemic in Utah. Most isolates are Shigella sonnei, making it difficult to identify epidemiologic clustering. To better define transmission, molecular markers and epidemiologic data were examined for 90 cases. Plasmid analysis and pulsed-field gel electrophoresis (PFGE) of the S. sonnei isolates identified 11 and 4 patterns, respectively. Plasmid pattern I infections occurred in 8 day care centers over a 6-month period, suggesting spread between centers. Plasmid pattern III was isolated from children at 3 additional centers and pattern IV was associated with another day care center, suggesting different outbreaks. By PFGE, plasmid groups I and XI appeared identical, as were plasmid groups II and V; plasmid group X had a unique pattern. Plasmid groups III, IV, and VII-IX were closely related PFGE subtypes. Both plasmid analysis and PFGE allow better characterization of S. sonnei transmission patterns of "endemic" strains and could lead to improved control measures.
志贺氏菌病在犹他州高度流行。大多数分离株为宋内志贺氏菌，这使得识别流行病学聚集情况变得困难。为了更好地确定传播情况，对90例病例的分子标记和流行病学数据进行了检查。宋内志贺氏菌分离株的质粒分析和脉冲场凝胶电泳（PFGE）分别鉴定出11种和4种模式。质粒模式I感染在6个月内发生在8个日托中心，表明在各中心之间传播。质粒模式III从另外3个中心的儿童中分离出来，模式IV与另一个日托中心有关，表明存在不同的疫情爆发。通过PFGE，质粒组I和XI看起来相同，质粒组II和V也是如此；质粒组X有独特的模式。质粒组III、IV和VII - IX是密切相关的PFGE亚型。质粒分析和PFGE都能更好地表征“地方性”菌株的宋内志贺氏菌传播模式，并可能导致改进控制措施。
