Pichel M, González Fraga S, Terragno R, Mulki J, Gentile A, Kremer C, Mola A M, Noseda R, Binsztein N
Departamento Bacteriología, Instituto Nacional de Enfermedades Infecciosas - ANLIS Carlos G. Malbran, Buenos Aires, Argentina.
Epidemiol Infect. 2007 May;135(4):681-7. doi: 10.1017/S0950268806007230. Epub 2006 Sep 26.
Thirty-five isolates of Shigella sonnei from patients with diarrhoea in three geographic regions of Argentina were examined for genetic diversity by pulsed-field gel electrophoresis (PFGE) and plasmid profile. PFGE of XbaI and BlnI DNA digests confirmed the occurrence of outbreaks in two regions caused by two separate predominant clones of S. sonnei. The third region was characterized by three circulating clones, one of which was possibly associated with an outbreak. Similar plasmids were found in distinct clones and in one outbreak clone five different plasmid profiles were identified. Antimicrobial resistance of the isolates varied from fully susceptible to the agents tested, to resistance to cotrimoxazole, ampicillin and ciprofloxacin. Antibiotic resistance did not correlate with plasmid content. This information will form the basis for active surveillance of shigellosis in Argentina and elsewhere in the region through the PulseNet International Network.
对从阿根廷三个地理区域腹泻患者中分离出的35株宋内志贺菌进行了脉冲场凝胶电泳（PFGE）和质粒图谱分析，以检测其遗传多样性。XbaI和BlnI DNA酶切的PFGE证实，在两个区域发生了由两种不同的宋内志贺菌优势克隆引起的暴发。第三个区域的特征是有三个流行克隆，其中一个可能与一次暴发有关。在不同的克隆中发现了相似的质粒，在一个暴发克隆中鉴定出五种不同的质粒图谱。分离株的抗菌耐药性从对所测试药物完全敏感到对复方新诺明、氨苄西林和环丙沙星耐药不等。抗生素耐药性与质粒含量无关。这些信息将成为通过国际脉冲网络对阿根廷及该区域其他地方的志贺菌病进行主动监测的基础。
