van Gool T, Vetter J C, Weinmayr B, Van Dam A, Derouin F, Dankert J
Department of Medical Microbiology, Academic Medical Center, University of Amsterdam, Netherlands.
J Infect Dis. 1997 Apr;175(4):1020-4. doi: 10.1086/513963.
Encephalitozoon species are important pathogens in human immunodeficiency virus-infected patients. However, in immunocompetent persons, little is known about Encephalitozoon infections, mainly because of the lack of reliable diagnostic tests. To improve diagnosis, three serologic techniques that use Encephalitozoon intestinalis as antigen were developed: an ELISA, an immunofluorescence technique (IFAT), and a counterimmunoelectrophoresis (CIE) method. The serologic response against E. intestinalis was studied in sera from 300 Dutch blood donors and 276 pregnant French women. For confirmation of specificity, sera from 150 subjects with various infectious and noninfectious diseases were examined. ELISA, IFAT, and CIE were specific for microsporidia infections, and IFAT and CIE were specific for Encephalitozoon infections. High antibody titers against Encephalitozoon organisms were found in 24 (8%) of 300 Dutch blood donors and in 13 (5%) of 276 pregnant French women. The high seroprevalence against Encephalitozoon species in Dutch blood donors and French women suggests that Encephalitozoon infection is common in immunocompetent subjects.
脑胞内原虫属物种是人类免疫缺陷病毒感染患者的重要病原体。然而，在免疫功能正常的人群中，人们对脑胞内原虫感染了解甚少，主要是因为缺乏可靠的诊断检测方法。为了改进诊断，开发了三种以肠脑炎微孢子虫为抗原的血清学技术：酶联免疫吸附测定（ELISA）、免疫荧光技术（IFAT）和对流免疫电泳（CIE）方法。对300名荷兰献血者和276名法国孕妇血清中针对肠脑炎微孢子虫的血清学反应进行了研究。为了确认特异性，检测了150名患有各种感染性和非感染性疾病的受试者的血清。ELISA、IFAT和CIE对微孢子虫感染具有特异性，IFAT和CIE对脑胞内原虫感染具有特异性。在300名荷兰献血者中有24名（8%）以及在276名法国孕妇中有13名（5%）发现了针对脑胞内原虫的高抗体滴度。荷兰献血者和法国女性中针对脑胞内原虫属物种的高血清阳性率表明，脑胞内原虫感染在免疫功能正常的人群中很常见。
