Dziedzic B, Walczewska A
Department of Physiology, Medical University of Lodz, Poland.
Exp Clin Endocrinol Diabetes. 1997;105(1):57-62. doi: 10.1055/s-0029-1211728.
The aim of the present study was to examine if superior cervical ganglionectomy (SCGx) modifies the gonadotropin-releasing hormone (GnRH) content in the median eminence in female rats. Intact, ovariectomized (OVX) and ovariectomized, 17 beta-estradiol implanted (OVX + E2) female rats were subjected to SCGx or sham operation (sham-SCGx). After 12 or 48 hours they were decapitated and the GnRH content in the median eminence was determined by radioimmunoassay. SCGx performed 12 hours earlier decreased GnRH content in the median eminence in cycling female rats (p < 0.05) as compared with sham-operated control. No changes in GnRH content were observed 48 hours after SCGx. Ovariectomy decreased GnRH content in the median eminence (p < 0.05) and SCGx caused a further decrease in GnRH content (p < 0.05). Implantation of 17 beta-estradiol suppressed the dramatic decrease in GnRH content in the median eminence observed after SCGx in OVX rats without estrogen treatment. After SCGx the GnRH content in OVX + E2 rats was significantly higher than in OVX rats (p < 0.05). Our present results demonstrate that SCGx has some transient influence on the GnRH content in the median eminence. It may be assumed that noradrenaline released from degenerating sympathetic neurons located at the superior cervical ganglia (SCG) affects the GnRH-ergic terminals in the median eminence. The data support the hypothesis that SCG have a modulatory role in the mechanisms controlling the function of the hypothalamic-hypophyseal axis.
本研究的目的是检验颈上神经节切除术（SCGx）是否会改变雌性大鼠正中隆起中促性腺激素释放激素（GnRH）的含量。将完整、去卵巢（OVX）以及去卵巢并植入17β-雌二醇（OVX + E2）的雌性大鼠进行SCGx或假手术（假SCGx）。12或48小时后将它们断头，通过放射免疫测定法测定正中隆起中GnRH的含量。与假手术对照组相比，提前12小时进行的SCGx使处于发情周期的雌性大鼠正中隆起中的GnRH含量降低（p < 0.05）。SCGx术后48小时未观察到GnRH含量的变化。去卵巢使正中隆起中的GnRH含量降低（p < 0.05），而SCGx导致GnRH含量进一步降低（p < 0.05）。植入17β-雌二醇抑制了未接受雌激素治疗的OVX大鼠在SCGx后观察到的正中隆起中GnRH含量的显著降低。SCGx后，OVX + E2大鼠的GnRH含量显著高于OVX大鼠（p < 0.05）。我们目前的结果表明，SCGx对正中隆起中GnRH的含量有一些短暂的影响。可以推测，从位于颈上神经节（SCG）的退化交感神经元释放的去甲肾上腺素会影响正中隆起中的GnRH能终末。这些数据支持了SCG在控制下丘脑-垂体轴功能的机制中具有调节作用这一假说。
