Jezová D, Tokarev D, Kostálová L, Strbák V
Institute of Experimental Endocrinology, Slovak Academy of Sciences, Bratislava, Slovakia.
Gen Physiol Biophys. 1996 Aug;15(4):333-8.
Maternal milk is a significant source of hormones and other bioactive substances. They might be involved either in the control of mammary gland function or in the regulation of growth and development of the neonate. Atriopeptin (atrial natriuretic factor, ANF) is a peptide with strong diuretic, natriuretic and vasorelaxant actions, and it has been suggested to play an important role in the circulatory adaptation to extrauterine life. The aim of this study was to determine whether ANF is present in maternal milk, using radioimmunological analysis. The levels of ANF-like substance in human milk were found to be in the range of 0.3-3.0 pg/ml, those in rat milk between 37-117 pg/ml. The measured concentrations of ANF were proportional to the volume of the extracted milk. Serial dilutions of the extracts yielded curves which were not totally parallel to the human alpha-ANF standard curve. Our data indicate that, during the first days after delivery, ANF levels in human milk are higher than those in later periods of lactation. This pilot study provides the first description of the presence of atriopeptin in milk. Though a detailed characterization of milk ANF-like immunoreactivity is needed, a biological significance of present findings seems possible.
母乳是激素和其他生物活性物质的重要来源。它们可能参与乳腺功能的控制，或新生儿生长发育的调节。心钠素（心房利钠因子，ANF）是一种具有强大利尿、利钠和血管舒张作用的肽，有人认为它在宫外生活的循环适应中起重要作用。本研究的目的是通过放射免疫分析确定母乳中是否存在ANF。人乳中ANF样物质的水平在0.3 - 3.0 pg/ml范围内，大鼠乳中的水平在37 - 117 pg/ml之间。测得的ANF浓度与提取的乳汁体积成正比。提取物的系列稀释产生的曲线与人类α-ANF标准曲线并非完全平行。我们的数据表明，在分娩后的头几天，人乳中的ANF水平高于哺乳期后期。这项初步研究首次描述了乳汁中的心钠素。尽管需要对乳汁中ANF样免疫反应性进行详细表征，但目前的发现似乎具有生物学意义。
