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毛滴虫原生动物的精细结构与同工酶特征

Fine structure and isozymic characterization of trichomonadid protozoa.

作者信息

Mattos A, Solé-Cava A M, DeCarli G, Benchimol M

机构信息

Laboratório de Ultraestrutura Celular Hertha Meyer, Universidade Federal do Rio de Janeiro, Brazil.

出版信息

Parasitol Res. 1997;83(3):290-5. doi: 10.1007/s004360050249.

DOI:10.1007/s004360050249
PMID:9089728
Abstract

Tritrichomonas suis and T. foetus are characterized herein at the ultrastructural and biochemical levels. Microcinematography and measurements, scanning and transmission electron microscopy, cytochemistry for carbohydrate detection (Thiéry technique), and isozyme electrophoresis analysis were performed. In all, 11 different strains from 5 species of parasites were studied (T. foetus, T. suis, Trichomonas gallinae, T. vaginalis, and Monocercomonas sp.). A total of 11 enzymes were scored. Fine-structure study using scanning and transmission electron microscopy demonstrated that T. suis and T. foetus are identical morphologically. The high degree of isozymatic similarity noted between T. suis and T. foetus is consistent with the hypothesis that they may be different strains of the same species.

摘要

本文在超微结构和生化水平上对猪三毛滴虫和胎儿三毛滴虫进行了表征。进行了显微电影拍摄和测量、扫描和透射电子显微镜检查、用于碳水化合物检测的细胞化学(蒂埃里技术)以及同工酶电泳分析。总共研究了来自5种寄生虫的11种不同菌株(胎儿三毛滴虫、猪三毛滴虫、鸡三毛滴虫、阴道毛滴虫和单鞭滴虫属)。共对11种酶进行了评分。使用扫描和透射电子显微镜进行的精细结构研究表明,猪三毛滴虫和胎儿三毛滴虫在形态上是相同的。猪三毛滴虫和胎儿三毛滴虫之间观察到的高度同工酶相似性与它们可能是同一物种的不同菌株这一假设一致。

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