Xu W D, Lun Z R, Gajadhar A
Centre for Animal Parasitology, Canadian Food Inspection Agency, Saskatoon, Saskatchewan.
Vet Parasitol. 1998 Aug 31;78(4):247-51. doi: 10.1016/s0304-4017(98)00150-2.
Tritrichomonas foetus and Tritrichomonas suis isolates were cultivated axenically in Diamond's medium. Studies on the chromosome numbers of these two species with a light microscope were done by adding different concentrations of colchicine into the medium, incubating at 37 degrees C for 6-8 h and using a hypotonic swelling technique. The diploid chromosome numbers of both T. foetus and T. suis were 2n=10.
胎儿三毛滴虫和猪三毛滴虫分离株在戴蒙德培养基中进行无菌培养。通过向培养基中添加不同浓度的秋水仙碱、在37℃下孵育6 - 8小时并使用低渗肿胀技术,利用光学显微镜对这两个物种的染色体数目进行了研究。胎儿三毛滴虫和猪三毛滴虫的二倍体染色体数目均为2n = 10。